SELDI-TOF-MS技术的改善和优化  

Improvement and Optimization of SELDI-TOF-MS

作　　者：陈雅斌[1] 兰小鹏[2] 

机构地区：[1]福建医科大学福州总医院临床医学院,中国福建福州350025 [2]福州总医院全军检验医学研究所,中国福建福州350025

出　　处：《生命科学研究》2010年第1期90-94,共5页Life Science Research

基　　金：南京军区医学科学技术研究"十一五"计划(06Z47)

摘　　要：表面增强激光解吸电离飞行时间质谱(Surface-enhanced laser desorption/ionization-time of flight,SELDI-TOF-MS)技术是目前蛋白质组学研究较为有效的手段之一,已被广泛的应用于肿瘤、传染性疾病、心血管病和神经系统等疾病的研究.然而SELDI-TOF-MS技术本身存在着一些问题,如分析结果有效性差和实验重复验证率低等,使其不能完全的应用于常规的临床诊断,现有许多研究致力于解决这些问题,如规范化实验室操作、预处理原始标本和数据、提高统计学方法等极大的改善和优化了这项技术.The surface-enhanced laser desorption/ionization-time of flight （SELDI-TOF-MS） is one of the most promising approaches in proteome research. Now it is widely used in some researches, such as cancer, infection disease, angiocardiopathy, nervous system disease, and so on. But some limitations in SELDI-TOF-MS, for example： the effectivity and duplicate validation of the experimental result is low. Further evaluation and optimization are called for before it＇s application in clinical diagnostics. A lot of researches such as standardize the experimental process, pretreatment of natural sample and data, improve statistic method can optimize this technology remarkably.

关 键 词：SELDI.TOF—MS技术:改善:优化 

分 类 号：R-331[医药卫生]