新疆伊犁地区动物脑组织博尔纳病病毒p24基因片段的检测  

The detection on the p24 gene fragment of Borna disease virus in brain tissues of animals in Ili, Xinjiang

作　　者：何丰[1,2] 冯裕星[1,2] 孙后超[1,2] 胡子成[1,2] 徐红波[1,2] 徐鸣明[1,2] 展群岭[1,2] 胡永波[1,2] 金戈[1,2] 张英英[1,2] 李雷雷[2] 谢鹏[1,2] 

机构地区：[1]重庆医科大学附属第一医院神经内科,400016 [2]重庆医科大学神经科学研究中心,400016

出　　处：《中华微生物学和免疫学杂志》2010年第1期31-35,共5页Chinese Journal of Microbiology and Immunology

基　　金：“十一五”国家高技术研究发展计划（863计划）项目（2006AA02Z196）

摘　　要：目的了解新疆伊犁地区动物脑博尔纳病病毒（Borna disease virus，BDV）感染现状，分析该病毒的种系来源，预防我国BDV大规模暴发流行。方法采用巢式逆转录实时荧光定量聚合酶链反应（FQ—RT-PCR）的方法，对新疆地区200匹马、75头驴和100只牧羊犬脑组织标本进行BDVp24基因片段的检测。并对阳性标本进行BDV p40基因片段扩增电泳，同时排除GFP—p24和pMD-19质粒污染，最后克隆测序，进行基因同源性和氨基酸序列分析并分析BDV种系发生。结果3例伊犁马、4例伊犁驴和9例牧羊犬的脑组织标本BDV p24检测阳性，阳性率分别为1．5％、5．3％、9．0％；BDV p40基因片段检测和质粒标准品GFP—p24、pMD-19检测均为阴性；BDV pPA扩增产物序列与He／80株的同源性为100％。结论新疆伊犁地区马、驴和狗可能存在BDV脑内的自然感染，该地区BDV流行株与He／80株存在同源性。Objective To observe the epidemiology characterization of Borna disease virus （BDV） in animal brain in Ili, Xinjiang, and to find out the potential infection of the Borna disease virus to prevent its outbreak. Methods The BDV p24 gene of animal brain tissues in Ili including 200 horses, 75 donkeys and 100 shepherd dogs was detected by fluorescence quantitative nest reverse transcriptase polymerase chain reaction（FQ-nRT-PCR）. GFP-p24, pMD-19 plasmid contamination was excluded from positive products. Clone sequencing was used to analyze the homology of gene and amino acid sequence. Results BDV p24 gene was found in 3 Ili horses, 4 Ili donkeys and 9 shepherd dogs, and the positive ratio is 1.5 %, 5.3% and 9.0%, respectively. The GFP-p24 ,pMD-19 were not found in BDV p40 gene and plasmid standard. The sequence of BDV p24 amplification production was totally the same as He/80 virus strain. Conclusion Natural infection of BDV may exist in the animals （ horses, donkeys and dogs ） in Ili, and the epidemic strain of BDV in this area was homological as He/80 virus strain.

关 键 词：博尔纳病病毒 巢式逆转录荧光定量PCR 脑组织 新疆伊犁地区 

分 类 号：R749.3[医药卫生—神经病学与精神病学] R382.31[医药卫生—临床医学]