兔角膜穿通伤合并海水浸泡后房水单核细胞趋化蛋白-1含量变化的研究  

Changes in the content of monocyte chemoattractant protenin-1 in aqueous humor following penetrating corneal trauma complicated with seawater immersion in rabbits

作　　者：左晶 范钦华 杨建东 刘宇 张志强 任德奎 

机构地区：[1]解放军第八一医院眼科 ,江苏省南京210002

出　　处：《中华航海医学与高气压医学杂志》2009年第6期337-339,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：南京军区医学科学技术研究“十一五”课题资助项目（06MA66）

摘　　要：目的观察爆炸导致兔角膜穿通伤合并海水浸泡后房水单核细胞趋化蛋白-1（monocyte chemoattractant protenin-1，MCP-1）含量的变化及意义。方法成年健康灰兔10只，以右眼为实验眼，左眼为对照眼。鞭炮致兔角膜爆炸伤后，于角膜中周部做长约3mm全层切口。将海水通过实验眼角膜切口注入前房，海水持续灌滴眼表30min。对照眼使用生理盐水。造模后第1、2、3、5、7天采用ELISA法测定房水中MCP-1含量。结果实验眼造模后第1、2、3天房水中MCP-1含量分别为247．018、143．939、93．637ng／L。对照眼分别为81．373、72．194、59．921ng／L。2组相比差异有统计学意义（P〈0．01）。结论爆炸导致的兔角膜穿通伤合并海水浸泡后早期房水MCP-1含量明显高于对照眼，提示MCP-1参与这一病理改变并发挥重要作用。Objective To investigate changes in the content of monocyte chemoattractant protenin-1 （MCP-1） in aqueous humor following penetrating corneal trauma induced by explosion and complicated with seawater immersion in rabbits, and also its importance in the mechanism involved. Methods The model of penetrating corneal trauma was developed with fire crackers by using 20 rabbits as subjects, with the right eye as the experimental eye and left eye as the control. The right eye was immersed with seawater and the left eye immersed with saline solution. Levels of MCP-1 in aqueous humor for the 2 groups were determined by enzyme -linked immunosorbent assay （ELISA） on the 1st, 2nd, 3rd, 5th and 7th days following development of the model. Results Levels of MCP-1 in aqueous humor for the experimental group were 247. 018 ng/L, 143. 939 ng/L, 93. 637 ng/L respectively on the 1st, 2nd, 3rd days following development of the model. Comparisons showed that statistical difference could be seen between the 2 groups （ P 〈 0.01 ）. Conclusions Levels of MCP-1 in aqueous humor following penetrating corneal trauma by explosion and complicated with seawater immersion were significantly higher than those of the control group, indicating that MCP-1 might be involved and have an important role to play in this pathological process.

关 键 词：角膜穿通伤 海水浸泡 单核细胞趋化蛋白 房水 

分 类 号：R77[医药卫生—眼科]