血清蛋白质指纹谱对结直肠癌根治术后复发转移的诊断价值  

Significance of serum protein fingerprinting for the diagnosis of relapsing and/or metastatic colorectal cancer after radical resection

作　　者：高春芳[1] 盛新华[1] 王秀丽[1] 赵光[1] 郑国宝[1] 李冬晖[1] 范乃军[1] 

机构地区：[1]解放军150中心医院全军肛肠外科研究所,河南洛阳471031

出　　处：《解放军医学杂志》2010年第2期126-129,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十一五"基金(08Z006)

摘　　要：目的应用血清蛋白质指纹谱筛选与结直肠癌根治术后复发转移相关的特异性蛋白标志物。方法收集结直肠癌根治术后经至少2年随访、明确有复发转移的141例患者手术前后的血清标本,以及无复发转移的109例患者手术前后的血清标本,应用表面增强激光解吸离子化飞行时间质谱仪(SELDI-TOF-MS)检测所有患者的血清蛋白质指纹谱,并采用化学发光法测定血清CEA含量。筛选出与术后复发转移相关的血清标志蛋白,应用ROC曲线评价其对结直肠癌根治术后复发转移的诊断效能并与CEA进行比较。结果分析显示,在根治术后出现复发转移的结直肠癌患者中,M/Z为7763的血清蛋白不仅在术前表达增高,在明确有复发转移时仍呈显著高表达。该蛋白预测复发转移的灵敏度为92.0%,特异度为83.9%,约登指数指数为0.759,而CEA预测复发转移的灵敏度为52.0%,特异度为82.3%,Youden指数0.343。结论M/Z为7763的血清蛋白可能是结直肠癌根治术后复发转移的特异性标志蛋白,有望用于术后复发转移的监测。Objective To screening the specific protein biomarkers associated with relapsing and/or metastatic colorectal cancer （CRC） after radical resection using serum protein fingerprinting.MethodsThe serum samples were collected of 141 patients with definite relapsing and/or metastatic CRC,as well as 109 patients without relapsing and/or metastatic CRC （followed-up for at least 2 years）.The serum protein fingerprinting was read by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS）,and the serum level of carcinoembryonic antigen （CEA） was determined by chemiluminescence assay.The serum protein biomarkers associated with relapsing and/or metastatic CRC after radical resection were screened out,and the diagnostic efficacy of the biomarkers was evaluated by receiver operating characteristic curve （ROC） and compared with that of CEA.ResultsIn the patients with relapsing and/or metastatic CRC after radical resection,the protein M/Z 7763 was highly expressed not only before operation but also at the time when definite recurrence and/or metastasis was diagnosed.The sensitivity,specificity and Youden index （YI） of M/Z 7763 protein for prediction of relapsing and/or metastatic CRC after radical operation were 92.0%,83.9% and 0.759,respectively,while of CEA were 52.0%,82.3% and 0.343,respectively.ConclusionThe protein M/Z 7763 may be one of specific protein biomarkers associated with relapsing and/or metastatic CRC after radical operation,and may have a potential value in postoperative monitoring.

关 键 词：结直肠肿瘤 蛋白质组学 光谱法 质量 表面增强激光解吸离子化飞行时间 肿瘤复发 局部 肿瘤转移 

分 类 号：R735.340.4[医药卫生—肿瘤]