聚乙烯醇对大鼠牙髓细胞矿化诱导能力的实验研究  

Differentiation and mineralization-inducing effect of polyvinyl alcohol on rat dental pulp cells

作　　者：庞楠[1] 邹敬才[1] 荀文兴[1] 张红梅[1] 王燕[1] 曲晓莉[1] 张秀丽[1] 杨海珍[1] 杨香菊[1] 陈静涛[1] 陈晓囡[1] 

机构地区：[1]第四军医大学唐都医院口腔科,陕西西安710038

出　　处：《牙体牙髓牙周病学杂志》2010年第1期6-9,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察聚乙烯醇(polyvinyl alcohol,PVA)对大鼠牙髓细胞的矿化诱导能力。方法:制备PVA涂层24孔板,分别以PVA、矿化诱导液对第4代SD大鼠牙髓细胞进行矿化诱导,通过定量的茜素红染色分析和碱性磷酸酶(ALP)活性检测比较二者的矿化诱导能力。结果:经PVA诱导的大鼠牙髓细胞,其ALP活性和茜素红分析在7 d的培养过程中,显示明显持续增高的趋势(P<0.01)。结论:PVA可诱导大鼠牙髓细胞矿化分化,有望成为组织工程化人工牙研究的支架材料。AIM： To investigate the mineralization inducing effect of polyvinyl alcohol （PVA） on dental pulp cells of SD rat . METHODS： Primary dental pulp cells of rat were cultured on non-coated （control）, non-coated plus mineralization solution and PVD-coated 24 well culture plates in DMEM. The mineralization of the cells was determined by measuring the alkaline phosphatase （ALP） activity and by Alizarin Red S （ARS） mineralization assay at 1,4 and 7d after induction. RESULTS： At all time points, both PVA and mineralization inducing solution significantly increased ALP activity and calcium sedimentation （ P 〈 0.01 ） of the cells. PVA showed much stronger differentiation inducing effect than the mineralization-inducing solution （P 〈 0.01 ）. CONCLUSION： PVA is able to induce the differentiation and mineralization of rat dental pulp cells and might be a suitable scaffold for the study of tissue engineering teeth.

关 键 词：聚乙烯醇 牙髓细胞 矿化诱导 茜素红染色 碱性磷酸酶 

分 类 号：R780.2[医药卫生—口腔医学]