野黄芩苷拮抗内毒素抑制人牙周膜细胞增殖作用的实验研究  

Effects of scutellarin on proliferation of LPS-treated human periodontal ligament cells

作　　者：杨宁[1] 张莹[2] 葛志华[3] 王春艳[1] 

机构地区：[1]承德医学院,河北承德067000 [2]第四军医大学口腔医学院,陕西西安710032 [3]承德医学院附属医院,河北承德067000

出　　处：《牙体牙髓牙周病学杂志》2010年第1期14-17,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察野黄芩苷对内毒素(LPS)抑制人牙周膜细胞增殖的影响。方法:原代培养人牙周膜细胞(hPDLC),采用MTT比色法,流式细胞技术观察野黄芩苷对LPS抑制人牙周膜细胞增殖活性和细胞周期的影响。结果:LPS浓度为100μg/mL时,人牙周膜细胞增殖活性受到明显抑制,加入0.001～10μg/mL不同浓度野黄芩苷,在不同时间点能促进人牙周膜细胞的增殖(P<0.05),其中野黄芩苷浓度为1μg/mL时促增殖作用最强。结论:野黄芩苷具有拮抗LPS抑制人牙周膜细胞增殖的作用。AIM： To examine the effects of scutellarin on the proliferation of lipopolysaccharide （ LPS）-treated human periodontal ligament cells （hPDLCs）. METHODS： Primary hPDLCs cultured in vitro were stimulated with 100μ g/mL LPS, and LPS plus various concentrations of scutellarin, hPDLCs without treatment served as controls. After 24 h, 48 h and 72 h incubation, MTT assay was performed to assess the effects of scutellarin on cell proliferation. Flow cytometry was used to measure the DNA content after 24 h of incubation. RESULTS ： LPS significantly inhibited the proliferation of hPDLCS. Seutellarin dose-dependently stimulated the growth of LPS-treated hPDLCs at different time points, and the optimal stimulating effect of scutellarin was 1 μg/mL （ P 〈 0.05 ）. CONCLUSION ： Scutellarin Can significantly promote the proliferation of LPS-treated hPDLCs.

关 键 词：野黄芩苷 人牙周膜细胞 内毒素 细胞增殖 细胞周期 

分 类 号：R781.4[医药卫生—口腔医学]