ω-3多不饱和脂肪酸对重症急性胰腺炎大鼠耐力和氧化应激的影响  

Effect of fish oil on the stamina and antioxidase in rats with severe acute pancreatitis

作　　者：陈震[1] 余震[1] 刘纳新[1] 陈笑雷[1] 章晓东[1] 历金雷[1] 黄卡特[1] 

机构地区：[1]温州医学院附属第一医院普通外科,浙江温州325000

出　　处：《肠外与肠内营养》2010年第1期32-35,40,共5页Parenteral & Enteral Nutrition

基　　金：吴阶平医学基金会资助(WJP01)

摘　　要：目的:观察重症急性胰腺炎(SAP)大鼠经ω-3多不饱和脂肪酸治疗后,大鼠的耐力和氧化应激的变化,探讨ω-3多不饱和脂肪酸对SAP的影响。方法:将36只大鼠随机分为SAP等渗盐水组(NSG),SAP鱼油治疗组(FOG)和对照组(CG)。NSG组和FOG组均应用3.5%牛磺胆酸钠逆行胰胆管注射法诱导SAP模型。模型制备后,NSG组和FOG组分别注射等渗盐水和ω-3多不饱和脂肪酸,连续注射7 d,并于第1、3、5和7天进行改良大鼠鼠尾悬吊试验,观察大鼠的耐力。于7 d后处死大鼠,观察大鼠血清淀粉酶、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)等浓度的变化和大鼠胰腺组织的病理变化。结果:FOG组大鼠的耐力明显优于NSG组,仅次于CG组。FOG组与NSG组比,GSH-PX活力明显升高,MDA活力明显降低,且MDA与改良大鼠鼠尾悬吊试验阈上面积及总静止时间有相关性。FOG组大鼠胰腺组织病理变化轻于NSG组。结论:SAP大鼠经ω-3多不饱和脂肪酸治疗后耐力明显增强,抗氧化水平有所改善。Objective： To investigate the stamina,the role of antioxidant system in severe acute pancreatitis（SAP）,and the effect of fish oil treatment in rats.Methods： Thirty-six male Sprague-Dawley rats（SD） were randomly divided into three groups,including SAP group（sodium chloride treatment）（group NSG,n=12）,fish oil treatment group（group FOG,n=12）,and control group（group CG,n=12）.SAP was induced by intergraded injection of 3.5% sodium tanrocholate to biliopancreatic duct of SD rats in group NSG and FOG.The group NSG rats were treated by subcutaneous injection of sodium chloride,while the group FOG rats were treated by subcutaneous injection of fish oil for 7 days.Then the improved tail suspension test was observed at the first,third,fifth,seventh day.12 rats in each group were respectively sacrificed after 7 day.The activity of serum antioxidant enzymes（MDA,GSH-PH） and the concentration of serum amylase were measured in each group,and the levels of threshold on the area and the total immobility time were measured in each group.The severity of pancreatitis was analyzed according to the histopathological morphology.Results： Compared to group NSG,the severity of pancreatitis was significantly decreased in group FOG.The activity of MDA was significantly increased in group NSG than that in group CG（P〈0.01）,while the activity of MDA in group FOG was decreased than that in group NSG（P〈0.05）.The activity of GSH-PH was significantly decreased in group NSG than that in group CG（P〈0.01）,while the activity of GSH-PH was increased in group GOG than that in group NSG（P〈0.05）.THE level of threshold on the area was decreased in group NSG than that in group CG（P〈0.01）,while the level of threshold on the area was increased in group GOG than that in group NSG.（P〈0.01） The total immobility time was significantly increased in group NSG than that in group CG（P〈0.01）,while the total immobility time was decreased in group GOG than that in group NSG（P〈0.05）.MDA was

关 键 词：Ω-3多不饱和脂肪酸 重症急性胰腺炎 耐力 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R576[医药卫生—消化系统]