氟西汀和丙米嗪对应激诱导的青幼期大鼠抑郁和焦虑样行为的不同影响  

Different effects of imipramine and fluoxetine on depression and anxiety-related behavior induced by stress in adolescent rats

作　　者：谢希[1,2] 邵枫[3] 罗晓敏[1,2] 管西婷[1,2] 孟庆轩[3] 王玮文[1] 

机构地区：[1]中国科学院心理研究所心理健康院重点实验室,北京100101 [2]中国科学院研究生院 [3]北京大学心理学系

出　　处：《中国神经精神疾病杂志》2010年第1期14-18,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金项目(编号:30500158;30670708);中科院创新工程重要方向项目(编号:KSCX2-YW-R-131);973项目(编号:2007CB512306);中科院心理研究所发展基金(编号:O9KF013001)

摘　　要：目的建立青幼期大鼠的慢性应激抑郁模型,并探讨不同类型抗抑郁药物对其抑郁和焦虑样行为表现的影响。方法青幼期大鼠(日龄30～50d)接受3周慢性温和应激,同时随机分别给予选择性5-羟色胺重摄取抑制剂氟西汀和三环类抗抑郁剂丙米嗪(10mg/kgi.p.)。采用蔗糖水偏好、旷场和高架十字迷宫测试评估大鼠的抑郁和焦虑样行为。结果与对照组(n=10)相比,慢性应激模型组(n=11)大鼠体质量增长减慢,糖水偏好指数降低,上述差异均有统计学意义(P<0.05)。丙米嗪干预组(n=10)的糖水偏好明显低于丙米嗪对照组(n=10),差异有统计学意义(P<0.05),而氟西汀干预组(n=11)与氟西汀对照组(n=11)相比则没有明显差异(P>0.05)。在旷场测试中,青幼期后期(PND52)正常对照组大鼠与其早期(PND29)相比旷场直立行为次数降低(P<0.05),但自发活动量(水平运动距离)没有明显差异(P>0.05),氟西汀干预明显增加大鼠直立行为次数(P<0.05)。此外,PND52正常对照组大鼠与PND29相比在高架十字迷宫开放臂的进入次数和停留时间减少,而在闭合臂停留时间增加;应激模型组与正常对照组相比开放臂进入次数增加,闭合臂停留时间减少,上述差异均有统计学意义(P<0.05)。结论慢性应激诱导了青幼期大鼠的抑郁样行为。氟西汀而非丙米嗪可逆转大鼠的抑郁样行为。应激降低了青幼期大鼠的焦虑水平,而两种药物对焦虑行为均没有明显作用。Objective To establish chronic stress model of depression in adolescent rats and to examine the effects of different antidepressant treatment on depression and anxiety-related behaviors. Methods Male Wistar rats were given 21-day chronic mild stress （CMS） during their adolescence （postnatal day 30 -50, PND30 -50）. During stress period, rats were treated with fluoxetine （ 10 mg/kg i. p. ） or imipramine （ 10 mg/kg i. p. ）, respectively. After stress, rats were tested for behavioral observation using body weight gain, saccharine test, open field and elevated plus-maze （EPM）. Results Compared with control/vehicle （ n = 10） group, stress/vehicle （ n = 11 ） group displayed lower weight gain, saccharine preference index and the number of rearing in open field （ P 〈 0. 05 ）. Antidepressant fluoxetine, but not imipramine reversed anhedonia and the decrease of the number of rearing induced by stress. In addition, compared wilh early adolescent （PND29） rats, late adolescent （PND52） rats in control/vehicle group exhibited less open atTn entries and open arm time, more closed arm time in EPM （P 〈 0. 05 ）. Rats in stress/vehicle group showed more open arm entries and less closed arm time than controls（ P 〈 0. 05 ）. Both fluoxetine and imipramine had no effects on such changes. Conclusions Stress can induce the depression-like behavior in adolescent rats. Fluoxetine, but not imipramine,can effectively reverse anhedonia induced by stress. However, Both antidepressants have no significant effects on stress-induced decrease in developmental increment of anxious behavior during adolescence. These data suggest that chronic mild stress have complicated effects on depressive and anxious behavior in adolescent rats.

关 键 词：抑郁 应激 青幼期 丙米嗪 氟西汀 

分 类 号：R749.4[医药卫生—神经病学与精神病学] R749.053[医药卫生—临床医学]