血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建  

Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion

作　　者：张贺楠[1,2] 齐岩[1,2,3] 史伟伟[1,2] 梁艺瑜[1,2] 刘洪波[1,2] 张小桃[1] 曹伟胜[1,2] 廖明[1,2] 

机构地区：[1]华南农业大学兽医学院/农业部动物疫病防控重点开放实验室,广东广州510642 [2]农业部动物疫病防控重点开放实验室,广东广州510642 [3]中国牧工商(集团)总公司,北京100070

出　　处：《中国预防兽医学报》2010年第2期94-97,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30771612);NSFC-广东联合基金(U0831002);广东省自然科学基金(8151064201000065);广东省科技计划项目(20090204)

摘　　要：为构建血管瘤病变型J亚群禽白血病病毒E元件缺失突变体病毒,本研究利用融合PCR方法将E元件缺失,获得含有缺失性突变体病毒前病毒全基因组的重组质粒pSCAU-HN-△E。将该重组质粒纯化后转染DF-1细胞,并连续传代,最终获得缺失病毒rSCAU-HN-△E。该毒株感染的DF-1细胞可被ALV-J特异性单克隆抗体JE9所识别,并且体外增殖性能稳定。该缺失性病毒的获得为E元件功能的研究奠定了基础。The Subgroup J avian leukosis virus （ALV-J） strain SCAU-HN06 isolated from commercial layer hens with spontaneous hemangiomas has a complete ＂E＂ element in 3＇UTR. In order to rescue the E element-deleted virus, we constructed the recombinant plasmid pSCAU-HN-△ E that contained the whole ALV-J genome but with E element deleted. The plasmid was transfected into DF-1 cells and the rescued virus was identified by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The recombinant virus was stable in cell culture during nine passages and had a titre up to 10^3.6 TCID50/0.2 mL.

关 键 词：J亚群禽白血病病毒 血管瘤 E元件 感染性克隆 

分 类 号：S852.65[农业科学—基础兽医学]