高强度聚焦超声消融兔VX2种植性乳腺肿瘤的病理学变化  

Pathological changes of VX2 implanted breast cancer ablated with high intensity focused ultrasound in rabbits

作　　者：牛陵川[1] 邹文兵[1] 张炼[2] 朱辉[2] 向理科[3] 王智彪[1,2] 

机构地区：[1]重庆医科大学生物医学工程系,重庆市生物医学工程学重点实验室,重庆市超声医学工程重点实验室-省部共建国家重点实验室培育基地,重庆400016 [2]重庆医科大学附属第二医院肿瘤治疗中心,重庆400010 [3]重庆医科大学病理学教研室,重庆400016

出　　处：《肿瘤》2010年第2期105-108,共4页Tumor

摘　　要：目的:观察高强度聚焦超声(high intensity focused ultrasound,HIFU)消融兔VX2种植性乳腺肿瘤后,"即刻"这一时间点的肿瘤组织病理学变化。方法:36只新西兰兔接受VX2肿瘤种植后2周,随机分为治疗组24只和对照组12只。治疗组在B型超声监控下接受HIFU消融治疗后即刻取材,HE染色后在光学显微镜下观察病理组织学变化,然后进行电子显微镜观察、琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性检测和肿瘤抗原性免疫组织化学检测。结果:组织学观察发现,HIFU消融后的靶区内肿瘤细胞形态和细胞核型无明显改变,细胞质出现空泡样变;电子显微镜观察显示,肿瘤细胞呈凝固性坏死;SDH活性检测显示肿瘤细胞失活;增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在对照组的肿瘤细胞内呈阳性表达,而在治疗组的肿瘤细胞中不表达。结论:VX2种植性乳腺肿瘤经HIFU消融后,组织学观察到看似正常的肿瘤细胞经电子显微镜、酶学和免疫组织化学检测证实已经死亡。Objective：To observe the pathological changes of VX2 implanted breast cancer ablated by high intensity focused ultrasound （HIFU） in rabbits. Methods：Thirty-six rabbits implanted VX2 breast carcinoma were randomly divided into treatment group （n=24） and control group （n=12） two weeks after implantation. HIFU ablation was performed in the treatment group guided by ultrasound B. The pathological changes were observed under light microscope after hematoxylin-eosin staining and then observed under electron microscope. The activity of succinate dehydrogenase （SDH） was measured. Proliferating cell nuclear antigen （PCNA） was determined by immunohistochemistry. Results：Histopathologic observation found that the morphological features and nucleus karyotypes of tumor cells had no significant changes,but vacuole-like structure appeared in cytoplasm. Typical coagulation necrosis of tumor cells were observed under electron microscope. SDH activity detection showed that tumor cells were inactivated. The expressions of PCNA was positive in control group and negative in treatment group. Conclusion：Electron microscopy,enzyme property test,and immunohistochemistry verified that VX2 implanted breast cancer cells were dead after HIFU ablation in rabbits.

关 键 词：乳腺肿瘤 超声 高强聚焦 肿瘤种植 增殖细胞核抗原 琥珀酸脱氢酶 VX2肿瘤 兔 

分 类 号：R737.9[医药卫生—肿瘤] R73-36[医药卫生—临床医学]