TGF-β1、wt-p53基因在前列腺癌组织中的表达及其临床意义  

Evaluation of the expression of wt-p53 and TGF-β1 in the tissue of prostate carcinoma and its clinical significances

作　　者：苏振波[1] 梁作文[2] 洪泉[3] 赵丹[2] 王伟华[1] 

机构地区：[1]吉林大学中日联谊医院泌尿外科,吉林长春130033 [2]吉林大学白求恩医学院病理生理学教研室 [3]解放军空军总医院泌尿外科

出　　处：《中国老年学杂志》2010年第3期289-291,共3页Chinese Journal of Gerontology

基　　金：国家科学技术部国际科技合作重点项目(2004DFB02000)

摘　　要：目的探讨TGF-β1、wt-p53基因在前列腺癌组织中的表达并探讨其临床意义。方法应用免疫印迹技术检测正常前列腺、前列腺癌组织和癌旁组织中TGF-β1,wt-p53基因的表达。结果正常前列腺外腺和癌旁组织间的TGF-β1、wt-p53表达水平无差异(P>0.05);前列腺癌组织中TGF-β1和wt-p53表达,与正常前列腺组织和癌旁组织相比差异显著(P<0.01);已有远处转移灶的前列腺癌组织中TGF-β1和wt-p53表达,与无转移灶者相比差异显著(P<0.01)。结论TGF-β1和wt-p53基因与前列腺癌的发生和转移相关,并可能在前列腺癌的进展中起重要作用。因此,借助测定TGF-β1与wt-p53表达可辅助前列腺癌的诊断和评估预后。Objective To detect the expressions of TGF-β and wt-p53 gene in prostate carcinoma and evaluate its significances. Methods Western blotting method was used to detect TGF-β and wt-p53 expression in the normal and earcinomous prostatic tissues. Results TGF-β1 was similarly expressed in the normal and prostatic paraneoplastic tissues （ P 〉 0. 05 ）, and so was wt-p53 （ P 〉 0. 05）. Either TGF-β1 or wt-p53 was differently expressed in the careinomous prostatic tissues compared with normal or paraneoplastie tissues（P 〈0. 01 ）. The expressions of TGF-β1 or wt-p53 were statistically different between metastatic prostate carcinoma and non-metastatic carcinoma（ P 〈0. 01 ）. Concmusions TGF-β1 and wt-p53 genes are related to the malignant transformation and metastatis of prostate carcinoma. To detect the expressions of TGF-β1 and wt-p53 may make for judging prostate cancer＇s diagnosis and prognosis.

关 键 词：前列腺癌 TGF-Β1 WT-P53 免疫印迹法 

分 类 号：R737.25[医药卫生—肿瘤]