快速老化小鼠P8肾脏衰老的病理特点  

The pathological characteristic of aging kidney in senescence accelerated mouse/prone 8

作　　者：施晓芸[1] 陈晓春[1] 石艳清[1] 杨明[1] 叶洪[1] 赵朝晖[1] 

机构地区：[1]福建医科大学附属协和医院福建老年医学研究所,福建福州350001

出　　处：《中国老年学杂志》2010年第3期327-329,共3页Chinese Journal of Gerontology

基　　金：国家重点基础研究发展规划项目基金(No2007CB507400);福建省自然科学基金(No2006J0343)

摘　　要：目的观察快速老化小鼠P8(SAMP8)肾脏病理结构改变。方法以9月龄SAMP8为衰老动物模型,同龄抗快速老化小鼠R1(SAMR1)为对照,通过PAS、Masson染色及衰老相关β-半乳糖苷酶(SA-β-gal)组织化学染色观察肾脏组织病理的改变,免疫组化方法比较肾小管间质细胞外基质(ECM)成分——纤维连接蛋白(FN)、Ⅲ型胶原(ColⅢ)的变化。结果9月龄SAMP8小鼠光镜下肾脏病理表现为小管间质纤维化、局灶节段性肾小球硬化等,以小管间质损害较为显著;SA-β-gal染色阳性细胞显著增加;免疫组化结果显示FN、ColⅢ在肾小管间质过度沉积。上述改变与SAMR1组相比,差异有显著性。结论SAMP8肾脏的病理改变符合肾脏衰老的特点。Objective To study renal pathological structural changes in senescence accelerated mouse P8 （SAMPS）. Methods SAMP8 （9 months） were used as senescence animal model and senescence aeeelerated mouse/resistance 1 （ SAMRI ） with same age as a control group. Aging kidney pathology was determined by several senescent indicators, sueh as PAS and Masson staining. SA-β-galaetosidase activity was measured by histochemistry staining and extraeellular matrix components （ fibroneetin and eollagen Ⅲ ） were measured by immunohistoehemieal staining. Results Compared with SAMR1 group, SAMP8 group appeared different levels of senescence, lRenal pathological structural changes in SAMP8 （9 months） , including tuhulointerstitial fibrosis and focal segmental glomeruloselerosis, were found through microscope, and there was significant injury in the tubulointerstitial. Positive staining for SA-β-galaetosidase was widely observed. And the expressions of fibroneetin and collagen Ⅲ increased renlarkably which indicating tubulointerstitial fibrosis. Conclusions Renal pathological changes in SAMP8 mice accord with the pathological characteristic of aging kidney.

关 键 词：快速老化小鼠 衰老 肾脏 

分 类 号：R361[医药卫生—病理学]