非人工通气兔主动脉瓣上狭窄模型的建立及评价  

A rabbit model of supravalvular aortic stenosis without endotracheal intubation

作　　者：赵哲[1] 陈林[1] 高顺记[2] 郑德志[1] 肖颖彬[1] 

机构地区：[1]第三军医大学新桥医院全军心血管外科中心,重庆400037 [2]第三军医大学新桥医院全军超声诊断中心,重庆400037

出　　处：《第三军医大学学报》2010年第5期427-430,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(CSTC2009BB5025)~~

摘　　要：目的探讨在非人工通气的条件下通过升主动脉缩窄的方法建立主动脉瓣上狭窄模型的可行性并进行评价。方法将20只新西兰大白兔完全随机分为升主动脉缩窄组(n=12)和假手术组(n=8)。在非人工通气的条件下,在主动脉近端根部结扎升主动脉,使其缩窄。假手术组只行开胸手术,不行升主动脉结扎。术后2、4、8、12、16、20、24周行超声心动图检查,测量心室内径及心功能。术后8、24周行光学显微镜及透射电子显微镜检查,观察心肌组织形态学改变。结果超声心动图检查示:升主动脉缩窄组术后8周时左室质量指数(left ventricular mass index,LVMI)明显增大[(2.30±0.11)mg/g],较术前[(1.02±0.05)mg/g]及假手术组[(0.98±0.01)mg/g]均有显著差异(P<0.01),左室短轴缩短率(fractional shortening,FS%)自术后4周始逐渐下降,术后24周时较术前降低了9.3%,有显著差异(P<0.01)。组织学检查示:心肌纤维排列紊乱,心肌细胞肥大,间质胶原纤维显著增生。结论通过非人工通气升主动脉缩窄的方法成功建立了兔主动脉瓣上狭窄的模型,该方法简单、有效、重复性好。Objective To endotracheal intubation. Methods establish an animal model of supravalvular aortic stenosis in rabbits without New Zealand white rabbits were randomly assigned to the aortic banding group （n = 12） and the sham-operation group （n = 8 ）. The former group underwent aortic banding with 3-0 Prolene contiguous to the aortic valve without endotracheal intubation. The sham-operation group received the same procedures but without actual ligation of the aorta. To investigate the effects of the surgical procedure on physiological parameters, echocardiography, histology and electron microscopy were carried out to assess the function and structure of hypertrophy at various time intervals. Results Banding of the ascending aorta caused the expected increase in aortic velocity. The pressure overload resulted in a robust left ventricular hypertrophic （LVH） assessed by transthoracic echocardiography, microscopic histology, and electron microscopy. At week 8 after ascending aorta banding, the LV mass index was increased significantly 2.30 - 0.11 mg/g at week 8 vs 1.02 ± 0.05 mg/g at baseline and O. 98 -＋ O. 01 mg/g in sham-operation group, P 〈 0. 01 ）. While fractional shortening （FS） was progressively declined after 4 weeks, and the decrease was 9.3% at week 24 compared to the baseline （P 〈0.01 ）. Conclusion These results demonstrate a simple, effective, and reproducible method of producing supravalvular aortic stenosis in rabbits without the need for endotracheal intubation and mechanical ventilation.

关 键 词：左心室肥大 主动脉缩窄 非人工通气 动物模型 兔 

分 类 号：R-332[医药卫生] R542.52