GasderminA3基因突变对小鼠毛囊β-catenin表达的影响  

Effect of GasderminA3 gene mutation on β-catenin expression in mouse hair follicle in vivo

作　　者：雷明星[1] 杨恬[2] 连小华[2] 杨力[1] 

机构地区：[1]重庆大学生物工程学院,国家"111计划"基地,重庆400044 [2]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2010年第5期442-445,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30872270);高等学校学科创新引智计划(111计划;B06023)~~

摘　　要：目的探讨GasderminA3(GSDMa3)基因突变前后β-catenin在毛囊中表达的变化。方法采用免疫组化S-P法、RT-PCR、Western blot等方法,检测出生后11d(毛囊生长期)、16d(毛囊退化早期)、18d(毛囊退化晚期)、24d(毛囊静止期)等各个时相点的GSDMa3基因突变鼠和C57BL/6(B6)鼠背皮毛囊β-catenin的表达表化。结果GSDMa3基因突变鼠和B6鼠β-catenin的表达均从11～24d逐渐减弱,但GSDMa3基因突变鼠β-catenin的表达在各个时期均强于B6鼠。在生长期,β-catenin表达于内外根鞘和毛母质细胞中,在GSDMa3基因突变鼠的表达强于B6鼠;在退化期,β-catenin主要表达于外根鞘和毛母质细胞中,在突变鼠的表达更强于B6鼠;在静止期,β-catenin主要表达在突变鼠的外根鞘细胞中,而在B6鼠的毛囊中表达很少。结论GSDMa3基因可能通过调节β-catenin的表达,影响毛囊的生长和发育。Objective To study the expression changes of β-catenin in the hair follicle before and after GasderminA3 gene mutation. Methods Using SP immunohistochemical staining, RT-PCR, Western blotting to detect the expression of β-catenin in the hair follicle in GSDMa3 mutant and C57BL/6 （B6） mice on postnatal 11 （anagen）, 16 （early catagen）, 18 （late catagen） and 24 d （telogen） respectively. Results The expression of β-catenin is gradually weakened from 11 d to 24 d in GSDMa3 mutation mice and B6 mice, but stronger expression was found in GSDMa3 mutation mice than in the B6 mice at different time points. In anagen, β-catenin was expressed in the inner and outer root sheath and hair matrix cells, with stronger expression in GSDMa3 mutant mice than in B6 mice. In catagen, β-catenin was mainly expressed in the outer root sheath and hair matrix cells, with more stronger expression in GSDMa3 mutant mice than in B6 mice. In telogen, β-catenin was expressed in the outer root sheath cells in the mutant mice while little in the hair follicle in B6 mice. Conclusion GSDMa3 gene affects the hair follicle growth and development, probably through regulating the expression of β-catenin.

关 键 词：GSDMa3 基因突变 毛囊 Β-CATENIN 小鼠 

分 类 号：R322.995[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]