高糖诱导人脐静脉内皮细胞内皮微粒释放并促进细胞凋亡  

High glucose induces release of endothelial microparticles in human umbilical vein endothelial cells and promotes cell apoptosis

作　　者：田刚[1] 任燕[1] 卢群[1] 鲁敏[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2010年第2期181-185,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30270555;30871042);陕西省科技攻关项目[(No.2005K-14-G6(2)]~~

摘　　要：目的探讨高糖对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)内皮微粒释放和细胞凋亡的影响。方法用不同浓度的葡萄糖(5.5 mmol/L、16.7 mmol/L和33.3 mmol/L)分别作用于培养成功的HU-VEC 0、12、24、48、72 h,设立正常糖浓度组(5.5 mmol/L葡萄糖)和高渗对照组(27.8 mmol/L甘露醇)作为对照。应用MTT比色法检测细胞的生存率;采用流式细胞仪分别检测HUVEC内皮微粒的释放水平以及细胞凋亡率。结果①MTT检测发现,33.3 mmol/L葡萄糖培养12 h时HUVEC的细胞数比为93%,24 h时为78%,48 h时为51%,72 h时为40%;与0 h组相比,12 h组细胞数比下降但无统计学意义(P>0.05);与12 h相比,24 h组4、8 h组和72 h组细胞数比显著下降,且呈时间依赖性减低(P均<0.05)。②设5.5 mmol/L正常糖浓度组培养48 h时的HUVEC细胞数比为100%,则高渗对照组HUVEC细胞数比为98%,与正常糖浓度对照组比较无统计学差异(P>0.05);16.7 mmol/L葡萄糖组和33.3 mmol/L葡萄糖组的细胞数比分别为79%和51%,均明显低于正常糖浓度组(P均<0.05),且前两组相比亦存在统计学差异(P<0.05)。③33.3 mmol/L葡萄糖组作用12 h时的HUVEC内皮微粒生成量较正常糖浓度对照组明显增加(P<0.05),24、487、2 h时增加更为显著,且呈现时间依赖性(P<0.01);与正常糖浓度对照组相比,高渗对照组12、24、487、2 h时其内皮微粒释放水平无统计学差异(P>0.05);④高糖诱导的内皮微粒释放水平与细胞凋亡率呈正相关(r=0.659,P<0.05)。结论高糖刺激诱导人脐静脉内皮细胞内皮微粒释放,促进细胞凋亡。Objective To explore the effects of high glucose on release of endothelial microparticles（EMPs） and cell apoptosis in human umbilical vein endothelial cells（HUVECs）.Methods HUVECs were incubated for 0h,12h,24h,48h and 72h,respectively,with different concentrations of glucose（5.5mmol/L,16.7mmol/L and 33.3mmol/L,respectively）.The cells cultured in the media containing glucose 5.5mmol/L and mannitol 27.8mmol/L served as control groups.The survival rate of cells was examined by MTT assay.The level of EMPs release and percentage of cell apoptosis were detected by flow cytometry.Results ① According to MTT detection,the survival rate of HUVECs was 93% when incubated for 12h with 33.3mmol/L,78% for 24h,51% for 48h,and 40% for 72h.Compared with that for 0h,the survival rate for 12h did not decline significantly（P〉0.05）.the survival rate reduced significantly for 24,48 and 72h,respectively,in the time-dependent manner（P〈0.05）.② If the survival rate was 100% when incubated in 5.5mmol/L glucose for 48h,then it was 98% in mannitol 27.8mmol/L with no significant difference（P〉0.05）.It was 79% in 16.7mmol/L glucose and 51% in 33.3mmol/L glucose,obviously lower than that in 5.5mmol/L glucose,and the two groups differed significantly（P〈0.05）.③ the level of EMPs release increased significantly in 33.3mmol/L glucose for 12,24,48 and 72h respectively,in the time-dependent manner.Compared with the normal glucose group,the level of EMPs release did not significantly differ in mannitol 27.8mmol/L for 12,24,48 and 72h,respectively（P〉0.05）.④ There was a significantly positive correlation between EMPs release and cell apoptosis（r=0.659,P〈0.05）.Conclusion High glucose induces release of HUVECs endothelial miccroparticles and promotes cell apoptosis.

关 键 词：葡萄糖 糖尿病 动脉粥样硬化 内皮微粒 内皮功能 

分 类 号：R363[医药卫生—病理学]