荧光原位杂交法与免疫组织化学法检测乳腺癌c-erbB-2表达的比较  

Comparison of immunohistochemistry and fluorescence in situ hybridization in detecting c-erbB-2 expression in breast cancer

作　　者：盛薇[1] 车向明[1] 单涛[1] 樊林[1] 李萌[1] 张倩[1] 高希涛[1] 

机构地区：[1]西安交通大学医学院第一附属医院普外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2010年第2期208-211,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：卫生部课题基金资助(WKJ 2007-3-001)~~

摘　　要：目的比较免疫组织化学法(I HC)和荧光原位杂交法(FISH)检测乳腺癌c-erbB-2状态的一致性。方法选取乳腺癌石蜡标本共50例,其中以I HC法检测c-erbB-2蛋白表达(+)10例、()20例、()20例。以FISH法检测上述乳腺癌细胞c-erbB-2基因扩增情况,分析比较两种方法差异性与相关性。结果在I HC检测乳腺癌c-erbB-2(+)和()时,FISH检测结果与其具有较好的一致性,总符合率为89.2%。在I HC检测c-erbB-2()时,FISH结果与其一致性较差,符合率仅为35.3%。结论I HC可以作为初步筛查c-erbB-2表达的首选方法;I HC检测乳腺癌c-erbB-2(+)和()时与FISH有良好的一致性。因I HC检测c-erbB-2表达()时与FISH结果的一致性较差,这类患者在应用赫赛汀治疗时应做FISH确诊。Objective To compare the consistency of immunohistochemistry（IHC） and fluorescence in situ hybridization（FISH） in detecting c-erbB-2 status in breast cancer tissues.Methods A total of 50 breast cancer paraffin embedded samples were selected,of which there were 10 cases of c-erbB-2 protein expression（＋）,20 cases of（＋＋） and 20 cases of（＋＋＋）.FISH was used to assess the amplification of c-erbB-2 gene,and SPSS 13.0 software was employed to analyze the difference and consistency between the two methods.Results IHC and FISH methods had a good consistency when detecting c-erbB-2（＋） and（＋＋＋） expressions in breast cancer tissues,with the coincidence rate of 89.2%.However,when IHC was used to test c-erbB-2（＋＋）,the result of FISH was quite different,with the coincidence rate of only 35.3%.Conclusion IHC is a preliminary method to detect c-erbB-2 status in breast cancer.IHC and FISH methods have a good consistency in detecting c-erbB-2（＋） and（＋＋＋） status in breast cancer tissues.As detection of c-erbB-2（＋＋）with IHC has a different result from FISH,such patients should receive FISH confirmation for herceptin therapy.

关 键 词：乳腺癌 c—erbB-2 免疫组织化学技术 荧光原位杂交技术 

分 类 号：R737.9[医药卫生—肿瘤]