从血液流变学的改变分析肾上腺素致血瘀证大鼠模型的建立  

Hemorheological Changes in Rat Model with Blood Stasis Syndrome Induced by Adrenaline

作　　者：赵玲[1] 魏海峰[1] 李雅莉[1] 张丽[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室

出　　处：《中西医结合心脑血管病杂志》2010年第2期188-190,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家重点基础研究计划-973计划基金项目(No.2003CB517104);北京市自然科学基金项目(No.7050001;7063079);北京市科技新星计划项目(No.2005A53;2006B37);国家自然科学基金项目(No.30500664);首都医学发展基金项目(No.首发03Ⅲ21);北京市科技计划项目(No.D0206001043191)

摘　　要：目的以肾上腺素(Adr)皮下注射法建立大鼠血瘀模型,对比不同的给药剂量、给药方式和给药周期对大鼠血液流变学的影响,确定最佳的血瘀证动物模型的建立方法。方法将实验大鼠随机分为6组,即空白对照组(不造模)、急瘀1d组(Adr1.8mg/kg皮下注射加冰水浸泡1d)、急瘀2d组(Adr1.8mg/kg注射加冰水浸泡2d)、慢瘀小剂量组(Adr0.1mg/kg注射7d)、慢瘀中剂量组(Adr0.3mg/kg注射7d)、慢瘀大剂量组(Adr0.9mg/kg注射7d)。用血液黏度仪检测全血黏度和血浆黏度;用红细胞变形/聚集仪检测红细胞变形和聚集指数;用血凝仪检测血浆纤维蛋白原含量。结果与对照组相比,慢瘀小、中剂量组可见大鼠全血黏度、纤维蛋白原含量明显升高(P<0.05或P<0.01);急瘀1d组可见低切变下全血黏度升高(P<0.05),急瘀1d组和急瘀2d组可见血浆黏度和纤维蛋白原增加(P<0.01),急瘀2d组和慢瘀大剂量组可见红细胞变形指数明显降低(P<0.05)。结论血瘀证动物模型成功与否与所用的肾上腺素剂量、给药周期和给药方式密切相关。Adr0.3mg/kg注射7d和Adr1.8mg/kg注射加冰水浸泡2d大鼠出现血液流变学变化更加明显,可分别作为气郁血瘀证和气滞血瘀证的动物模型。Gbjective To testify the optimal way of using adrenaline（Adr） to establish animal model with acute or chronic Blood stasis syndrome. Methods Sprauge - Dawley rats were divided into 6 groups：Blank control group,one day acute model group（subcutaneous injection of Adr 1.8 mg/kg and soakage in ice water for one day）,two day acute model group（Adr 1.8 mg/kg s. e. and soak- age in ice water for two days）, low dose chronic model group （Adr 0. 1 mg/kg s. e. for consecutive 7 days）, middle dose chronic model group （Adr 0.3 mg/kg s. c. for 7 days） ,and high dose chronic model group （Adr 0.9 mg/kg s. c. for 7 days）. Blood viscosity,plasma fibrinogen,red blood cell（RBC）deformability and aggregation in rats were measured. Results Compared with blank control group,the whole blood viscosity and the plasma fibrinogen were increased in low dose and middle dose chronic groups（P〈0.05 or P〈0.01）. The whole blood viscosity at low shear rate were increased in one day acute model group （P〈0. 05）. The plasma viscosity and the plasma fibrinogen were increased in one day and two day acute model groups （P〈0.01）. The RBC deformability index was decreased in two day acute model and high dose chronic model groups （P〈0.05）. Conclusion Hemorrheologic changes were more significant in Adr 0.3 mg/kg s. c. for 7 days （middle dose chronic model group） ,Adr 1.8mg/kg s. c. and soakage in ice water for two days （two day acute model group）. These might be taken as the animal model for syndromes of Qi - depression with blood stasis and Qi stagnation with blood stasis respectively.

关 键 词：肾上腺素 血液流变学 血瘀证 大鼠 

分 类 号：R-332[医药卫生]