Humanin对缺氧诱导的大鼠皮层神经元损伤的保护作用  

The Neuroprotective Effects of Humanin on Hypoxia-induced Cortical Neurons Insults in Rats

作　　者：赵珅婷[1] 赵丽[2] 乔健天[3] 张策[3] 

机构地区：[1]广州医学院 [2]北京体育大学 [3]山西医科大学

出　　处：《中西医结合心脑血管病杂志》2010年第2期197-199,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金资助项目(No.30572085)

摘　　要：目的观察Humanin(HN)对缺氧诱导皮层神经细胞损伤的保护作用。方法培养10d的原代皮层神经元在缺氧环境下(氧含量<2%)放置4h诱导神经细胞损伤。实验组在缺氧之前24h分别加入终浓度为10μmol/L和20μmol/L的HN。通过测定MTT和Calcein-AM染色检测细胞活力。通过测定丙二醛(MDA)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)的变化检测细胞损伤的程度。结果HN可以提高缺氧4h后细胞的存活率,同时可以降低由细胞损伤所引起的MDA、SOD、LDH的升高。20μmol/L的HN具有比10μmol/L更显著的保护作用。结论HN对缺氧诱导的神经元细胞损伤具有保护作用。Objective To study the neuroprotective effects of humanin on the hypoxia -induced cortical neurons insults. Methods Primary cortical neurons were cultured for 10 days and then were put in the hypoxic environment（oxygen concentration 〈2%） for 4 h. Humanin was added to culture medium at 10 μmol/L and 20 μmol/L final concentration at 24 h before treatment. The cell survival rate was calculated by MTT assay and Calcein- AM staining. The production of malonaldehyde （MDA）, the viability of superoxide dismutase（SOD） and lactate dehydrogenase（LDH） were assayed. Results Humanin could improve the cell viability after hypoxia for 4 h and prevented hypoxia- induced the increase of levels of MDA,SOD and LDH. 20 μmol/L humanin showed a stronger neuroprotective effect than 10 μmol/L humanin did. Conclusion Humanin had a neuroprotective effect on hypoxia - induced cortical neurons insults.

关 键 词：HUMANIN 缺氧 MTT Calcein—AM 丙二醛 超氧化物歧化酶 乳酸脱氢酶 

分 类 号：R363[医药卫生—病理学] R285.5[医药卫生—基础医学]