葫芦素B体外对乳腺癌细胞的生长抑制作用研究  

Inhibitory effect of cucurbitacin B on the growth of breast cancer cells

作　　者：张美侠[1] 杨姣[1] 王艳丽[1] 周雪莹[1] 李岭[2] 邓意辉[3] 

机构地区：[1]中国医科大学药学院临床药理教研室,沈阳110001 [2]中国医科大学药学院遗传学教研室,沈阳110001 [3]沈阳药科大学药剂教研室,沈阳110015

出　　处：《实用药物与临床》2010年第1期7-9,共3页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省自然科学基金资助项目(20082092);留学回国人员科研启动基金(教外司[2006]331号)

摘　　要：目的观察葫芦素B(Cucurbitacin B)对非磷酸化STAT3人乳腺癌细胞系MCF-7和磷酸化STAT3人乳腺癌细胞系MDA-MB-435S的增殖抑制和诱导凋亡作用,为临床应用提供依据。方法用不同浓度的葫芦素B分别处理MCF-7和MDA-MB-435S细胞,48 h后用MTT法检测细胞增殖;用荧光显微镜观察细胞凋亡。结果MTT结果显示,葫芦素B对MCF-7和MDA-MB-435S细胞的增殖抑制作用具有明显的剂量依赖性,统计学分析提示,各实验组与对照组之间差异有统计学意义(P<0.05);荧光显微镜显示典型的细胞凋亡。结论葫芦素B对人乳腺癌细胞的增殖具有抑制作用(与STAT3的活化状态无关),诱导乳腺癌细胞凋亡,从而发挥抗肿瘤效应。Objective To investigate the inhibitory effect of cucurbitacin 13 on different human breast cancer ceils. Methods In vitro anti-proliferative activity of cucurbitacin B was investigated on MCF-7 and MDA-MB-435S ceil lines by MTT assay. Morphologic changes were evaluated under light microscope. Apoptotic cells were evaluated with Hoechst 33258 staining. Results Cueurbitacin B inhibited both STAT3 positive and STAT3 negative breast cancer cells proliferation in a dose dependent manner. Marked morphological changes of cell apoptosis including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly seen on Hoechst 33258 staining. Conclusion Cucurbitacin B can inhibit breast cancer cell growth independent upon STAT3 status.

关 键 词：葫芦素B 乳腺癌 MCF-7 MDA—MB-435S 凋亡 

分 类 号：R737.9[医药卫生—肿瘤] R735.2[医药卫生—临床医学]