JKA97诱导HepG2凋亡及其分子机制研究  

Effect of JKA97 in inducement of apoptosis of HepG2 cells and its molecular mechanism

作　　者：陈香郡[1] 张文斌[1] 陈耀明[1] 刘明朝[1] 车红磊[1] 赵芳[1] 姚婷[1] 孟姗姗[1] 马金龙[1] 王基野[1] 李丹[1] 骆文静[1] 

机构地区：[1]第四军医大学劳动与环境卫生学教研室,陕西西安710032

出　　处：《现代生物医学进展》2009年第23期4419-4421,F0002,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30471434)

摘　　要：目的:研究药物JKA97对人肝癌细胞HepG2增殖的抑制作用及其分子机制。方法:采用MTT法观察药物JKA97对细胞增殖的影响;倒置显微镜观察细胞形态变化;流式细胞术检测细胞凋亡;Western blot方法检测线粒体融合蛋白mfn2表达水平变化。结果:药物JKA97抑制人肝癌细胞HepG2细胞增殖,5、10、20μmol/L作用组24h抑制率分别为34.26%、43.08%、54.02%;药物JKA97诱导人肝癌细胞HepG2凋亡,10、20μmol/LJKA97作用HepG2细胞24h,细胞凋亡率分别为22.9%、72.9%;线粒体融合蛋白mfn2表达水平显著降低。结论:药物JKA97对人肝癌细胞HepG2生长具有明显的增殖抑制作用,诱导细胞凋亡;线粒体融合蛋白mfn2表达水平降低可能是其重要的分子机制之一。Objective：To investigate the inhibitory effect of JKA97 on proliferation and the molecular mechanism of human liver cancer cell line HepG2 in vitro.Methods：The cell viability was detected by MTT[3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyl tetrasoliumBromide];Morphological changes of HepG2 cells were measured by microscope;JKA97 induced apoptosis of HepG2 cells was detected by AnnexinV-PI staining and flow cytometry;The expression of mitochondria-shaping protein,mitofusin-2（mfn2）was measured by Western blot.Results：JKA97 induced the apoptosis of HepG2 cells and the expression level of mfn2 decreaed gradually. Conclusion：JKA97 can obviously inhibit the proliferation of human liver cancer cell line HepG2 in vitro and induce apoptosis of HepG2 cells.Lower expression of mitochondrial fusion protein mfn2 may be one of JKA97＇s molecular mechanisms.

关 键 词：JKA97 HEPG2 增殖抑制 凋亡 MFN2 

分 类 号：R735.7[医药卫生—肿瘤]