Fe(III)Cit还原菌的分离及其性能的优化  

Isolation and Characterization of Strain for Fe(III)Cit Reduction

作　　者：蔡灵琳[1] 姜锦林[1] 刘楠[1] 李伟[1] 

机构地区：[1]浙江大学环境工程研究所,浙江杭州310027

出　　处：《高校化学工程学报》2010年第1期156-161,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：国家“863”高技术研究发展计划资助项目(2006AA06Z345);国家自然科学基金资助项目(20676120);浙江省科技计划重点项目(2006C23064)

摘　　要：从化学吸收．生物还原集成系统内的混合培养物中分离得到一株高效还原Fe（Ⅲ）Cit（Cit：柠檬酸）的菌株，对其进行分子生物学鉴定，归属为肠球菌属，命名为Enterococcussp．FR．3。同时，考察了碳源种类和浓度、氮源种类和浓度、菌种接种量以及pH等因素对FR．3生长及Fe（Ⅲ）Cit还原性能的影响，优化微生物生长及还原Fe（Ⅲ）Cit的条件。结果表明，葡萄糖为FR．3生长和Fe（Ⅲ）Cit还原的最佳碳源，其最适添加浓度为1000mg·L^-1,NH4Cl作为氮源时菌种FR-3生长和Fe（Ⅲ）Cit还原效果明显优于NaNO2，且NHCl只要维持100mg．L-1左右即可。菌种接种量在150mg·L-1时Fe（Ⅲ）Cit还原率达到最大。过量碳源、氮源用量以及或接种量对还原过程没有促进作用。适合Fe（Ⅲ）Cit还原的pH值范围为6．6—7．0。A strain which can reduce Fe （Ⅲ）Cit （Cit： citrate） efficiently was isolated from the mixed culture of chemical absorption-biological reduction integrated system. Through molecular biological identification, the strain belongs to enterococcus, and was named to be Enterococcus sp. FR-3. The effects of carbon source species, nitrogen source species, the inoculation amount of cells and pH-value on the strain FR-3 growth and its Fe（Ⅲ）Cit reduction ability were investigated respectively. The results demonstrate that the glucose is the most suitable carbon source for the strain FR-3 growth and Fe（Ⅲ）Cit reduction, and the optimal glucose adding amount is 1000 mg.L-（When using NHaCl as nitrogen source, FR-3 growth and Fe（Ⅲ）Cit reduction efficiency are better than that of using NaNO2, and the optimal NHaCl adding amount is 100 mg.L-1, The bio-reduction of Fe （Ⅲ）Cit can be achieved efficiently with the cells inoculation amount of 150 mg-L-1, and the reduction rate would not further increase with adding more amount of cells inoculation. The optimal pH range found for Fe（Ⅲ）Cit reduction is 6.6-7.0.

关 键 词：生物还原 Fe(Ⅲ)Cit NO ENTEROCOCCUS sp. 

分 类 号：X172[环境科学与工程—环境科学] X701.7