大鼠骨髓间充质干细胞体外诱导分化为肝细胞(英文)  

DIFFERENTIATION OF MARROW BONE MESENCHYMAL STEM CELLS INTO HEPATOCYTES FROM RAT IN VITRO

作　　者：王忠琼[1] 夏世萍[1] 刘彦[1] 钟晓琳[1] 李昌平[1] 

机构地区：[1]泸州医学院附属医院消化内科,四川泸州646000

出　　处：《泸州医学院学报》2010年第1期40-44,共5页Journal of Luzhou Medical College

摘　　要：目的:研究HGF、aFGF及OSM诱导MSCs成为肝细胞的能力及其在分化中的作用。方法:用HGF、aFGF、OSM诱导分化MSCs后,用化学发光法、RT-PCR法及谷氨酸脱氢酶法检测细胞AFP、ALB及尿素的表达情况。结果:除对照组外其余各组细胞均有AFP表达(P<0.05);对照组及a+O组无ALB、尿素的表达,其余各组出现ALB及尿素的表达(P<0.05)。结论:HGF联合aFGF、OSM及aFGF能够诱导MSCs分化为表达AFP、ALB、尿素的肝细胞;aFGF与OSM在没有HGF存在时不能诱导MSCs向较为成熟的肝细胞的分化。Objectives：To study the potential of hepatocyte growth factor （HGF）, acid fibroblast growth factor （aFGF） and oncostatin M （OSM） to induce MSCs differentiate into hepatocytes and their roles. Methods：MSCs were treated with HGF, aFGF and OSM. Detecting the expression of AFP, ALB and urea was performed by chemi-luminescent technique, RT-PCR method and glutamic acid dehydrogenase technique. Results：AFP expression emerged in every group except control group （P〈0.05）. ALB and urea expression emerged in other groups except control group and a＋O group （P〈0.05）. Conclusion： HGF, OSM and aFGF can induce MSCs differentiate into hepatocytes with the expression of AFP, ALB and excreting urea, while aFGF and OSM can not induce MSCs differentiate into mature hepatocyte without HGF.

关 键 词：骨髓间充质干细胞 肝细胞生长因子 酸性成纤维细胞生长因子 制瘤素M 肝细胞 

分 类 号：R734.2[医药卫生—肿瘤]