一株高产果胶酶菌的分离及其产酶条件的优化研究  

Study on Isolation of A high-yield pectinase strain and Optimization of Fermentation Conditions

作　　者：王军[1] 黄秀琴[2] 王洁[2] 曾庆福[1] 陈悟[1] 陈洪高[1] 

机构地区：[1]武汉科技学院纺织印染清洁生产教育部工程研究中心,湖北武汉437300 [2]武汉科技学院环境与城建学院,湖北武汉430073

出　　处：《安徽农业科学》2009年第36期17902-17904,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅优秀中青年科技人才项目(Q20081704);武汉科技学院青年基金项目(20073204);中国纺织工业协会项目(2006074);国家科技支撑计划(2006BAC02A11)

摘　　要：[目的]挖掘新的高效产果胶酶菌株,为优化苎麻生物脱胶技术奠定基础。[方法]从沤麻池的厌氧泥、腐败的麻、苎麻园土壤中分离高产果胶酶菌株,在果胶平板上筛选、复筛选其中生长速度快、透明圈大、高产果胶酶的菌株,对其发酵培养基的初始pH值、发酵温度、发酵时间及接种量进行优化。[结果]获得了8株高产果胶酶的菌株,其中HY11产果胶酶活最高;该菌株在pH值为6、温度为35℃、时间为30 h、2.0%菜籽饼为碳源、1.0%NH_4H_2PO_4为氮源的培养基中为最佳发酵条件,果胶酶活力达到453 U/mL,比优化前提高了33.52%。[结论]获得了高产果胶酶菌株HY11,优化后的条件大大提高了其产酶活力。[Objective]The aim was to screen new efficient microbial strain for producing pectinase and provide the basis for optimizing the bio-degumming technology of ramie.[Method]Strains producing pectinase from ramie retting mud,putrefactive ramie and ramie planting soil were isolated,the high-pectinase strain with larger transparent circle which grew faster on pectin plate media was screened and re-screened. The initial pH value of fermentation medium,fermentation temperature,fermentation time,carbon source and nitrogen source of fermentation medium were optimized.[Result]Eight high-yielding strains had been obtained,of which HY11 had the highest activity in producing pectinase. Under the best fermentation conditions of pH value of 6,temperature of 35℃,time of 30 h,2.0%rapeseed cake as carbon source, 1.0%NH_4H_2PO_4 as nitrogen source of fermentation medium,the activity of pectinase reached 453 U/ml,which increased 33.52%compared with that before optimization.[Conclusion]High-yielding strain of pectinase named HY11 had been obtained,and its enzyme activity were considerably increased under the optimized conditions.

关 键 词：果胶酶 分离 发酵条件 

分 类 号：S182[农业科学—农业基础科学]