大豆胚轴体外诱导结肠癌细胞分化的研究  

Effect of Soybean Hypocotyls on Differentiation of Colon Carcinoma Cell HT-29 in vitro

作　　者：全吉淑[1] 许惠仙[2] 李天[1] 尹学哲[2] 

机构地区：[1]延边大学基础医学院,吉林延吉133000 [2]延边大学附属医院,吉林延吉133000

出　　处：《大豆科学》2010年第1期121-123,共3页Soybean Science

基　　金：吉林省科技发展计划资助项目(200705428);国家自然科学基金资助项目(30360113)

摘　　要：用免疫细胞化学法检测增殖细胞核抗原Ki-67和PCNA蛋白表达,分光光度法测定碱性磷酸酶(ALP)和乳酸脱氢酶(LDH)活性,ELISA法测定癌胚抗原(CEA)水平,研究了富含大豆异黄酮和皂甙的大豆胚轴提取物(SHE)对结肠癌细胞增殖和分化的影响。结果表明:富含大豆异黄酮和皂甙的大豆胚轴提取物可时间和浓度依赖性地降低结肠癌细胞Ki-67和PCNA表达,增加细胞ALP活性。CEA水平和LDH活性呈增高趋势,但差异不具有统计学意义。表明富含大豆异黄酮和皂甙的大豆胚轴可抑制结肠癌细胞增殖和诱导细胞分化,从而发挥抗结肠癌作用。Soybean isoflavones and saponins have been observed to induce the differentiation of various tumor cells. The effects of soybean hypocotyl extract（ SHE）, rich in isoflavones and saponins, on the proliferation and differentiation of colon carcinoma HT-29 cells were investigated. The immunocytochemical method was used for the determination of Ki-67 and PCNA, the spectrometric method was used for the detection of alkaline phosphatase（ALP） and lactate dehydrogenase （LDH）activities,the ELISA method was used for the assay of CEA. Results showed that SHE reduced the expression of Ki-67 and PCNA, and increased the ALP activity of colon carcinoma cells in a time- and concentration-dependent manner. However, no significant treatment effect was found for CEA and LDH levels. It is suggested that soybean hypocotyl, rich in isoflavones and saponins, could exert an anticolon cancer effect via inhibition of cell proliferation and induction of cell differentiation.

关 键 词：大豆 胚轴 异黄酮 皂甙 结肠癌 增殖 分化 

分 类 号：R735.3[医药卫生—肿瘤]