姜黄素对肝癌Hep1细胞增殖及凋亡的影响  

Effect of curcumin on the proliferation and apoptosis of hepatocellular carcinoma Hep1 Cells

作　　者：倪琦[1] 曾思恩[1] 谭宁[2] 郭芳[1] 张美艳[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]桂林医学院生物技术学院

出　　处：《山东医药》2010年第7期6-8,共3页Shandong Medical Journal

基　　金：广西壮族自治区研究生教育创新计划资助项目(桂学位[2008]14号)

摘　　要：目的进一步探讨姜黄素的抗肿瘤作用机制。方法CCK-8法检测不同浓度姜黄素作用不同时间肝癌Hep1细胞的增殖抑制率;流式细胞术检测Hep1细胞凋亡率以及各周期时相变化。结果姜黄素作用后Hep1细胞增殖抑制率明显升高,呈时间剂量效应关系;15μmol/L作用后,G0+G1期细胞显著增多,而G2+M期细胞显著减少,细胞凋亡率增高(P均<0.05)。结论姜黄素可有效抑制Hep1细胞的生长和增殖,且具有明显的量效关系;其机制可能为干扰细胞周期进程,促进细胞凋亡。Objective To explore the anti-tumor mechanism of cureumin furtherly. Methods The proliferation inhi- bition rate of Hepl cells treated with different concentrations of eureumin for different time was observed by CCK-8 method, the apoptosis and cycle phase of which was measured by FCM. Results The proliferation inhibition rate of Hepl cell sig-nifieantly increased after the treatment of eurcumin in a dose-dependent manner. After the treatment of 15 μmol/L eureumin, cells in G0 ＋ G1 phase increased significantly, while cells in G2 ＋ M phase reduced significandy and apoptosis rate in- creased （P 〈0.05）. Conclusions Curcumin can effectively inhibit the cell growth and proliferation of Hepl in a clear dose-response relationship, and the mechanism may be that cureumin can interfere cell cycle progression and promote cell apoptosis.

关 键 词：肝癌 姜黄素 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]