雷公藤内脂醇对SW480细胞的生长抑制作用  

Inhibition of triptolide on the growth of human colorectal carcinoma cell line SW480.

作　　者：孙鹏达[1] 房学东[1] 朱甲明[1] 

机构地区：[1]吉林大学白求恩第二医院,吉林长春130041

出　　处：《中国热带医学》2010年第3期287-288,共2页China Tropical Medicine

基　　金：吉林省发改委基金项目(2008-156)

摘　　要：目的观察雷公藤内酯醇(Tritolide,TL)对体外培养的SW480细胞(人结直肠癌细胞)的诱导分化机制,为人结直肠癌的治疗提供理论依据。方法应用噻唑蓝(MTT)比色法检测不同浓度的TL对SW480细胞的增殖抑制率,流式细胞仪检测SW480细胞周期进程及凋亡率,相差显微镜观察不同浓度的TL对SW480细胞形态学改变,电子显微镜观察细胞超微结构的变化。结果不同浓度的TL对SW480细胞增殖均有抑制作用,具有明显的剂量依赖性,流式细胞仪结果显示,G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,细胞凋亡率升高,相差显微镜下实验组细胞贴壁不佳,细胞数减少,细胞圆缩,电子显微镜下细胞高度空化,线粒体肿胀,可见凋亡小体。结论TL对SW480细胞有明显的增殖抑制作用,阻止SW480细胞G1期向S期的转化进程并诱导SW480细胞凋亡及超微结构改变。Objective To investigate the mechanism of inducing differentiation of human colorectal carcinoma cell line SW480 in vitro by triptolide in order to provide reliable theory for treatment of human colorectal carcinoma. Methods MTT assay was applied to detect the proliferation inhibition rate of SW480 by treatment with various concentration of triptolide .The cell cycle and apoptosis rate were assessed by flow cytometry. The morphologic change of SW480 ceils were observed by contrast phase microscope . The ultra-structure of SW480 cells was detected by electron microscope. Results Triptolide showed significant inhibiting effect on proliferation of SW480 cell in a dose-dependent manner. Flow cytometry showed the cells increased in G1 stage,decreased in S stage and relative increased in G2/M stage. In addition,the cells apoptosis rate increased. Contrast phase microscope examination indicated that the experimental cells had bad adherence, the number decreased and cell become shrunk. Electron microscope showed cell cavitation, mitochondria swelling and apoptotic body visualized. Conclusion Triptolide can obviously inhibit the proliferation of SW480 cell and hold back the course of transforming from G1 stage to S stage in addition to inducing SW480 apoptosis and changing cells ultra-structure.

关 键 词：SW480细胞 细胞周期 凋亡 TL 

分 类 号：R735.3[医药卫生—肿瘤]