SO_2胁迫对桑树部分生理生化特性的影响  

Effects of SO_2 Stress on Several Physiological and Biochemical Properties of Mulberry Trees

作　　者：窦宏伟[1] 周菲[1] 谢清忠 王维乐[1] 高绘菊[1] 王彦文[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]泰安丝绸集团有限公司,山东泰安271000

出　　处：《蚕业科学》2010年第1期126-131,共6页ACTA SERICOLOGICA SINICA

基　　金：山东省科技攻关项目(No.2007GG20009014);现代农业产业技术体系建设专项(蚕桑);公益性行业(农业)科研专项(No.nyhyzx07-020-08)

摘　　要：为探究桑树对SO2污染的抗逆机制,以盆栽实生桑苗为试验材料,采用密闭箱静态熏气法研究不同浓度SO2胁迫对桑树主要逆境生理生化特性的影响。桑树叶片中的含硫量随SO2胁迫处理浓度的增加而升高,随着SO2胁迫的加强,桑树叶片逐渐出现坏死,甚至萎蔫。桑树叶片过氧化氢酶(CAT)活性在处理前期随SO2胁迫处理浓度的增加而升高,处理后期当SO2质量浓度达到50 mg/m3之后,又表现出下降的趋势;桑树叶片的超氧化物歧化酶(SOD)活性则随SO2胁迫处理浓度的增加而急剧下降;在较低质量浓度(10、30 mg/m3)SO2胁迫下,桑树叶片的过氧化物酶(POD)活性表现出较大幅度的升高,而较高质量浓度(50 mg/m3)SO2胁迫下则表现出下降的趋势;桑树叶片的叶绿素含量、净光合速率随着SO2胁迫处理浓度的提高表现出减小趋势,脯氨酸、丙二醛(MDA)、可溶性蛋白质的含量则随SO2胁迫处理浓度的提高而增大。试验结果表明,SO2胁迫抑制了桑树保护酶活性,导致植株逆境伤害加剧,叶片产生坏死,降低了光合能力,对桑树生长产生不利影响。In order to understand the mechanism of mulberry＇s resistance to SO2 pollution,potted mulberry seedlings were placed in an enclosed chamber and added with SO2 with different concentrations to study the effects of SO2 on the main physiological and biochemical properties of mulberry.The results showed that sulfur content in mulberry leaves increased with the concentration of SO2 stress in the chamber.As the SO2 stress was enhanced,necrotic areas and even wilting occurred to the leaves gradually.The activity of catalase（CAT） in mulberry leaves increased with SO2 level in earlier stage and decreased in later stage after SO2 level reached 50 mg/m3.The activity of superoxide dismutase（SOD） in mulberry leaves decreased rapidly as SO2 stress was enhanced.Under relatively lower SO2 stress（10,30 mg/m3）,the activity of peroxidase（POD） in mulberry leaves increased substantially and declined under relatively high SO2 stress（50 mg/m3）.As SO2 stress was enhanced,chlorophyll content and net photosynthetic rate of mulberry leaves increased while proline,malonaldehyde and soluble protein contents decreased.Activity of protective enzyme was inhibited by SO2 stress which led to more serious injury,and larger necrotic areas on the leaves while photosynthetic rate declined.All these changes had negative influences on the growth and development of mulberry.

关 键 词：二氧化硫 桑树 逆境生理 酶活性 光合作用 丙二醛 脯氨酸 可溶性蛋白质 

分 类 号：S888[农业科学—特种经济动物饲养]