桑枝皮醇提物的抗氧化和对α-糖苷酶活性的抑制作用  

The Antioxidation and Inhibitory Effects of Mulberry Bark Ethanol Extract on α-Glucosidase Activity

作　　者：马永雷[1] 张雨青[1] 周丽霞[1] 卢立真[1] 李琳[1] 贺天真[1] 周珍祯[1] 阎海波[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,苏州市蚕丝生物技术重点实验室,江苏苏州215123

出　　处：《蚕业科学》2010年第1期143-147,共5页ACTA SERICOLOGICA SINICA

基　　金：国家高技术研究发展计划“863”项目(No.2006AA10-A118);现代农业产业技术体系建设专项(蚕桑)

摘　　要：α-糖苷酶活性抑制剂是一种治疗糖尿病的口服药物。以醇水溶液为萃取溶剂,从桑树枝条的皮中获得一种具有抑制α-糖苷酶活性的桑枝皮醇提物。桑枝皮醇提物的体外生物活性试验显示其对1,1-二苯基苦基苯肼自由基具有良好的清除作用,抑制中值(IC50)为100μg/mL;对酪氨酸酶、麦芽糖酶和蔗糖酶的活性均具有很强的抑制作用,IC50值分别为0.44 mg/mL、6.5μg/mL和0.25μg/mL。对桑枝皮醇提物的酶动力学分析显示其属于底物竞争性的α-糖苷酶抑制剂。研究结果初步表明,桑枝皮醇提物不仅具有一定的抗氧化作用,而且可作为一种新型的α-糖苷酶抑制剂用于糖尿病的血糖控制。α-Glucosidase inhibitor is a new type oral hypoglycemic drug for diabetes.In this experiment,ethanol was used as the extraction solvent to prepare mulberry bark extract that had the inhibitory effect on α-glucosidase activity.Biological activities of the mulberry bark ethanol extract were investigated.The results showed that the extract had a good scavenging activity to 1,1-diphenyl-2-picrylhydrazyl（DPPH） radicals with around 100 μg/mLof IC50 value.In vitro assay results revealed that the extract strongly inhibited tyrosinase,maltase and sucrase activities whose IC50 values were 0.44 mg/mL,6.5 μg/mL and 0.25 μg/mL,respectively.Enzyme kinetic analysis showed that the extract was an α-glucosidase inhibitor characteristic of substrate competition.Therefore,the extracts could be used as a novel α-glucosidase activity inhibitor for controlling blood glucose level in diabetes.

关 键 词：桑枝皮 醇提物 Α-糖苷酶抑制剂 1 1-二苯基苦基苯肼自由基 

分 类 号：R285[医药卫生—中药学]