乳铁蛋白修饰的前体阳离子脂质体的构建与表征  

Construction and characterization of lactoferrin modified procationic liposomes

作　　者：陈华黎[1] 巫孟君[1] 王雪[1] 唐蕾[1,2] 尹愈佳[1] 唐洁[1] 何勤[1] 

机构地区：[1]四川大学靶向药物与释药系统教育部重点实验室,成都610041 [2]成都军区总医院药剂科,成都610083

出　　处：《中南药学》2010年第2期81-84,共4页Central South Pharmacy

基　　金：国家重点基础研究发展计划课题(编号:2007CB935801)

摘　　要：目的构建并验证乳铁蛋白修饰的前体阳离子脂质体。方法以带有二硫键的胆固醇衍生物——[2-[[4-[(羧甲基)二硫]-1-亚氨丁基]氨基]乙基]氨基甲酸胆固醇酯(CHETA)代替传统骨架材料胆固醇,利用薄膜分散-超声法制备前体阳离子脂质体(procationic liposome,PCL),通过静电作用在PCL表面吸附乳铁蛋白(lactoferrin,Lf),得到了不同Lf/CHETA质量比例的Lf-PCL,并对Lf-PCL从形态、粒径、zeta电位、血清中的稳定性及Lf吸附量等方面进行表征。结果所制得的Lf-PCL体系形态规则,平均粒径范围为101.8~144.1 nm,多分散指数的PDI范围在0.175~0.254。Zeta电位的分布范围为-36.6^-2.2 mV,在血清中具有较好的稳定性,当Lf/CHETA质量比增加到8时,吸附趋于饱和。结论本文成功构建了乳铁蛋白修饰的前体阳离子脂质体(Lf-PCL)。Objective To construct and characterize a novel delivery system lactoferrin modified procationic liposomes, Methods A novel cholesterol derivative with a disulfide bond-CHETA was used to prepare the procationic liposomes （PCL） by thin film ultrasonic method, and lactoferrin （Lf） was adsorbed onto the surface of the PCL via electrostatic interaction. The Lf modified PCL （Lf-PCL） complex with different weight ratios was characterized in terms of diameter, zeta potential, serum stability and the amount of Lf adsorbed onto the surface of the PCL. Results The average diameter of the prepared LI-PCL complex was 101. 84144.1 rim, with the PDI between 0. 175-0. 254. The zeta potential was between -36.6--2.2 mV, and they were stable in the serum. When the weight ratio of Lf/CHETA reached 8, the adsorption reached saturation. Conclusion This study constructs Lf-PCL successfully.

关 键 词：前体阳离子脂质体 乳铁蛋白 表征 稳定性 

分 类 号：R944.9[医药卫生—药剂学]