细胞毒性T淋巴细胞和细胞因子诱导杀伤细胞在荷人胃癌裸鼠体内的迁徙与分布  

Evaluation of in vivo labeled cytotoxic T lymphocytes and cytokine-induced killer cells migration and distribution of subcutaneous gastric tumor model in nude mice

作　　者：武增瀛[1] 杜晓辉[1] 徐迎新[1] 李力[1] 刘巨超[1] 王金晶[1] 李则学[1] 

机构地区：[1]解放军总医院普通外科,北京100853

出　　处：《中华医学杂志》2010年第6期403-406,共4页National Medical Journal of China

基　　金：国家自然科学基金（60601018）

摘　　要：目的研究细胞毒性T淋巴细胞（CTL）和细胞因子诱导杀伤（CIK）细胞在荷人胃癌裸鼠体内的迁徙与分布特点。方法采用皮下注射人低分化胃腺癌细胞株BGC-823的方法建立荷人胃癌裸鼠模型。将荷瘤裸鼠分为2组，每组9只，分别经尾静脉输入^99Tc^m标记的CTL和CIK细胞。输入后1、6和24h，每组各取3只裸鼠，应用单光子发射型计算机体层摄影术（SPECT）观察2种细胞在裸鼠体内的分布状况，之后处死动物取肿瘤和肝、脾、肾、肺、肠等器官，以井型探测器测量每克组织百分注入活度（％ID／g），计算肿瘤组织％ID／g与正常组织％ID／g的比值（T／NT）。结果SPECT示踪显示2种细胞输入裸鼠体内后均迅速分布至肿瘤和肝、脾、肾、肺、肠等器官。CTL的％ID／g峰值由高到低依次为肿瘤（7．79±0．46）、肝（4．12±0．51）、肠（2．71±0．16）、肾（1．44±0．25）、脾（1．24±0．12）和肺（1．12±0．11），各组织器官T／NT值均〉1；CIK细胞的％ID峰值由高到低依次为肝（6．64±0．67）、肿瘤（5．47±0．87）、肠（3．55±0．23）、肾（2．34±0．41）、脾（1．45±0．17）和肺（1．27±0.21），除肝以外，其他各组织器官T／NT值均〉1。细胞输入后1、6和24h在肿瘤组织的％ID／g值，CTL分别为2．35±0．28、4．58±0．52和7．79±0．46，CIK细胞分别为2．23±0．46、3．25±0．70和5．47±0．87，24h时间点CTL组明显高于CIK组（P〈0．05）。结论CTL和CIK细胞在荷瘤裸鼠体内的分布均具有明确的肿瘤靶向性。Objective To investigate the migration and distribution of CTL （cytotoxic T lymphocyte） and CIK （cytokine-induced killer） cells in gastric tumor model. Methods Subcutaneous gastric tumor model was established by BGC-823 cancer cells in nude mice. Both CTL and CIK cells were labeled with ^99Tc^m directly and then inoculated into nude mice with subcutaneous tumor by intravenous injection separately. Three mice of each group were evaluated by single-photon emission computerized tomography （SPECT） at 1 h, 6 h and 24 h post-inoculation. After SPECT imaging,3 mice in each group were sacrificed and got samples of the tumor, liver, spleen, kidney, lung, intestine, etc. The tissue samples were weighed and radioactivity was determined with a well-type scintillation counter. The accumulation of labeled CTL and CIK cells in tissues were expressed as % ID/g （ percentage activity of injection dose per gram of tissue ） and T/NT （tumor/non-tumor） values were analyzed. Results The tracing of both cells in SPECT showed a clear migration path away from the injection point to solid tumor, and can be detected in all organs and tissues such as liver, spleen, kidney, lung and intestine, etc not long after injection. The % ID/g peak values of CTL in organs from the highest to the lowest were as follows： tumor （7.79±0.46）, liver （4. 12 ±0.51）, intestine （2.71 ±0. 16）, kidney （1.44 ±0.25）, spleen （1.24 ±0. 12）, kidney （1.12 ±0. 11）, and all the T/NTs were above 1. The %ID/g peak values of CIK cells in organs from the highest to the lowest were as follows ： liver （ 6. 64 ± 0. 67 ）, tumor （ 5.47 ± 0. 87 ）, intestine （3.55 ±0.23）, kidney （2.34±0.41）, spleen （1.45 ±0.17）, lung （1.27 ±0.21）, andT/NTs 〉 1 except for liver. After injection, the % ID/g values of tumor in CTL group were 2. 35 ± 0. 28 （ 1 h）, 4.58±0. 52 （6 h） and 7.79±0. 46 （24 h） respectively while the % ID/g values of tumor in CIK group 2. 23 ±0. 46 （ 1 h）, 3.25 ±0. 70 （6

关 键 词：胃肿瘤 肿瘤 实验性 T淋巴细胞 细胞毒性 杀伤细胞 体层摄影术 发射 型计算机 单光子 

分 类 号：R735.2[医药卫生—肿瘤]