转突变型淀粉样蛋白前体双荧光融合基因阿尔茨海默病小鼠模型的建立及初步鉴定  

Transgenic mice of Alzheimer＇ s disease： developing and preliminary identification of transgenic mice harboring mutant amyloid precursor protein gene fused with two types of fluorescent protein genes

作　　者：殷小平[1] 柴竞艳[1] 石元洪[2] 张苏明[2] 许杰[2] 李晓晴[2] 杨华静[2] 

机构地区：[1]九江学院附属医院神经内科,江西省九江市332000 [2]华中科技大学同济医学院附属同济医院神经内科

出　　处：《中华神经科杂志》2010年第2期135-138,共4页Chinese Journal of Neurology

摘　　要：目的探索建立转突变型淀粉样蛋白前体（APP）双荧光融合基因（CFP-54bp-YFP-C99）转基因小鼠模型的可行性和基因整合效率。方法采用显微注射法，将构建的突变型APP双荧光融合基因注入昆明小白鼠的受精卵，获取子代鼠，通过聚合酶链反应（PCR）方法初步鉴定基因的整合情况，以获得阳性鼠。结果注射昆明小白鼠受精卵共2202枚，移植1806枚。受体鼠56只，怀孕鼠13只，共生产52只子代鼠，其中存活32只。PCR初步检查整合有突变型APP双荧光融合基因的阳性鼠2只，均为活鼠。移植鼠的总怀孕率为23．2％（13／56），突变型APP双荧光融合基因的整合效率为3．9％（2／52）。结论显微注射法建立转突变型APP双荧光融合基因的阿尔茨海默病小鼠模型是可行的，但转基因效率较低。Objective To develop transgenic mice harboring the fusion gene of mutant amyloid precursor protein and two types of fluorescent protein for the future study on Alzheimer＇ s disease. Methods The fusion gene CFP-54 bp-YFP-C99 was introduced into mice by mieroinjeetion. The presence of CFP-54 bp-YFP-C99 was confirmed by PCR in the founders. Results CFP-54 bp-YFP-C99 gene was injected into pronueleus of 2202 zygotes and 1806 injected eggs were implanted into 56 foster mothers, 13 of which were pregnant. There were 13 foster mothers who borne 52 offspring and 32 of them survived. Recipient mouse pregnancy rate was 23.2% （13/56） and the integration rate was 3.9% （2/52）. Conclusion CFP-54 bp- YFP-C99 transgenic mice is obtained, but the transgenic efficiency is low.

关 键 词：阿尔茨海默病 小鼠 转基因 淀粉样Β蛋白前体 基因融合 绿色荧光蛋白质类 发光蛋白质类 

分 类 号：R749.16[医药卫生—神经病学与精神病学]