生存素、半胱氨酸天冬氨酸蛋白酶3在口腔癌前病变及口腔癌中的表达  

Expression of survivin, caspase-3 in oral precancerous lesions and oral squamous-cell carcinoma

作　　者：丁艳平[1] 李曙霞[1] 吴洪儒[2] 张辛燕[3] 汤晓飞[2] 孙正[1] 

机构地区：[1]首都医科大学口腔医学院牙周黏膜科,100050 [2]首都医科大学口腔医学院病理科,100050 [3]首都医科大学口腔医学院口腔医院研究所,100050

出　　处：《中华口腔医学杂志》2010年第2期85-88,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30500559）;北京市科技新星计划（2005B51）

摘　　要：目的探讨生存素（survivin）、半胱氨酸天冬氨酸蛋白酶3（caspase-3）在口腔癌发生中的作用，以期为口腔癌的监测和治疗提供参考。方法选取首都医科大学口腔医学院病理科存档石蜡标本45例，其中口腔自斑伴上皮轻-中度异常增生16例，口腔白斑伴上皮重度异常增生12例，口腔高．中分化鳞状细胞癌17例；正常口腔黏膜组织10例。采用免疫组化SP法对生存素、easpase-3的表达进行检测；脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法对细胞凋亡指数进行检测。结果生存素表达在正常黏膜、白斑伴上皮轻-中度异常增生、白斑伴上皮重度异常增生及口腔癌中逐渐增加，阳性细胞率分别为（1．05±1．21）％、（6．06±4．87）％、（12．49±8．41）％和（21．89±10．45）％；caspase-3的表达存3个病例组中逐渐下降，正常对照、自斑伴上皮轻-中度异常增生、白斑伴上皮重度异常增生及口腔鳞状细胞癌组阳性细胞率分别为（12．37±5．48）％、（19．51±13．15）％、（9．76±7．83）％和（6．08±6．91）％；正常对照、白斑伴轻-中度异常增生、白斑伴重度异常增生及口腔鳞状细胞癌组凋亡指数分别为（0．89±0．46）％、（1．294-0．63）％、（0．65±0．40）％和（0．21±0．12）％，前3组与口腔鳞状细胞癌组相比差异有统计学意义（P〈0．05）。结论生存素在口腔癌的发生过程中起重要作用，随着生存素表达的增加，caspase-3和凋亡指数呈下降趋势；生存素可能通过抑制caspase-3的表达，从而抑制细胞的凋亡，促进口腔癌的发生。Objective To investigate the roles of surviving and caspase-3 in the development of oral cancer. Methods Archival tissue sections of 17 oral squamous cell carcinoma（ OSCC）, 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme （TdT） mediated d-UTP end labeling （TUNEL）. Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis. Results The expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was （ 1.05 ± 1.21 ） % in control group and （21.89 ± 10. 45）% in OSCC. Caspase-3 was expressed in all the normal mucosa,but it obviously down- regulated in dysplasia and OSCC. The apoptosis index （ AI ） decreased from （ 0. 89 ± 0. 46 ） % in normal mucosa to （0. 21 ± 0. 12 ）% in OSCC. Conclusions Both survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting easpase-3.

关 键 词：半胱氨酸天冬氨酸蛋白酶3 口腔肿瘤 生存素 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]