不同转移潜能骨肉瘤细胞差异表达基因的筛查  

Screening of differentially expressed genes in osteosarcoma cell lines with various metastatic potentialities

作　　者：李新志[1] 孟琳[1] 陈安民[2] 郭风劲[2] 罗正强[2] 曾衡[2] 

机构地区：[1]三峡大学仁和医院骨科,湖北宜昌443001 [2]三峡大学医学院,湖北宜昌443000

出　　处：《中国肿瘤生物治疗杂志》2010年第1期71-76,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的:研究不同转移潜能骨肉瘤细胞差异表达的基因,筛选骨肉瘤转移相关的基因。方法:分别提取低转移骨肉瘤细胞株M6和高转移骨肉瘤细胞株M8细胞总RNA,采用cDNA基因芯片筛查M6和M8细胞差异表达的基因,杂交信号用GenerationⅢarray扫描,结果用Imagequant5.0软件分析。选择基因芯片筛选到的典型差异表达基因用实时定量PCR进行验证。结果:M6和M8骨肉瘤细胞株差异表达的基因共有330个,其中与低转移组(M6)相比,在高转移组(M8)中显著性上调表达的基因有178个,下调表达的基因有152个。其中发生非常显著性上调表达的基因43个,发生非常显著性下调表达的基因49个。差异基因主要包括与细胞增殖相关的基因,提示与M8细胞增殖受抑相关;其次是与基因表达调控及信号转导相关的基因,提示这些基因和肿瘤转移有关联性。结论:基因芯片方法对于筛选骨肉瘤转移相关基因有独特的优势,MG63细胞M8亚株中筛查到显著性上调的基因43个,显著性下调的基因49个,与骨肉瘤的转移有关。Objective：To investigate the differentially expressed genes in osteosarcoma cell lines with various metastatic potentialities,and to screen for new candidate genes related to metastasis of osteosarcomas.Methods：The total RNAs of a lowly metastatic and a highly metastatic osteosarcoma cell lines （M6 and M8） were extracted.Differentially expressed genes in the two osteosarcoma cell lines were studied by cDNA microarray.The hybridization signals were scanned with a Generation Ⅲ array scanner and analyzed by Imagequant 5.0 software.Typical differentially expressed genes were further verified by real-time quantitative PCR.Results：There were 330 differentially expressed genes between M6 and M8 cells.In the high-metastasis M8 cells,178 genes were up-regulated and 152 genes were down-regulated compared to the low-metastasis M6 cells,with 43 extremely up-regulated and 49 extremely down-regulated.The differentially expressed genes were mainly associated with cell proliferation,indicating these genes might be related to the inhibition of M6 cells.Other differentially expressed genes included those associated with the regulation of gene expression and signal transduction,indicating these genes might be correlated with tumor metastasis.Conclusion：cDNA microarray shows an advantage in identifying genes associated with metastasis of osteosarcoma.In M8 subset of MG63 osteosarcoma cells,43 genes are up-regulated and 49 genes are down-regulated,which may be related with metastasis of osteosarcoma.

关 键 词：骨肉瘤转移 CDNA芯片 基因表达谱 

分 类 号：R738.1[医药卫生—肿瘤] R730.2[医药卫生—临床医学]