神经生长因子促进MIA PaCa-2人胰腺癌细胞的增殖  

Nerve growth factor promotes proliferation of human pancreatic cancer cell line MIA PaCa-2

作　　者：孟令新[1] 丁兆军[1] 陈希平[1] 张红[1] 迟玉华[1] 

机构地区：[1]济宁医学院附属日照市人民医院肿瘤科日照市肿瘤治疗中心,山东日照276826

出　　处：《中国肿瘤生物治疗杂志》2010年第1期88-92,共5页Chinese Journal of Cancer Biotherapy

基　　金：日照市人民医院博士科研启动基金资助项目(No.080901)~~

摘　　要：目的:观察神经生长因子-β(nerve growth factor-beta,NGF-β)对人胰腺癌MIAPaCa-2细胞增殖及细胞周期的影响。方法:体外培养MIAPaCa-2细胞,单独或联合给予不同浓度的NGF-β和K252a(NGF-β受体TrKA的抑制剂),应用克隆平板实验、MTT法和流式细胞术检测NGF-β和K252a对MIAPaCa-2细胞克隆形成率、增殖及细胞周期的影响。结果:NGF-β显著促进MIAPaCa-2细胞的克隆形成(P<0.05),NGF-β使MIAPaCa-2细胞增殖能力明显增强(P<0.01),K252a抑制MIAPaCa-2细胞增殖(P<0.05),NGF-β与K252a联合作用对MIAPaCa-2细胞的增殖能力无明显影响。NGF-β作用使MIAPaCa-2细胞周期阻滞于S期,K252a作用使其周期阻滞于G0/G1期,两者联合作用使MIAPaCa-2细胞周期阻滞于S期。结论:NGF-β具有促进胰腺癌MIAPaCa-2细胞增殖的作用。Objective：To investigate the effect of nerve growth factor-β（NGF-β） on the proliferation and cell cycle of human pancreatic cancer MIA PaCa-2 cells.Methods：MIA PaCa-2 cells were treated with different concentrations of NGF-β and K252a （inhibitor of NGF-β receptor TrKA） alone or in combination.Clone forming rate,proliferation,and cell cycle of MIA PaCa-2 cells treated with different strategies were examined by clone formation assay,MTT,and flow cytometry,respectively.Results：NGF-β significantly increased the clone formation and proliferation of MIA PaCa-2 cells （P〈0.05,P〈0.01）.K252a significantly inhibited the proliferation of MIA PaCa-2 cells （P〈0.05）,while NGF-β combined with K252a had no significant effect on the proliferation of MIA PaCa-2 cells.NGF-β arrested MIA PaCa-2 cell cycle in S phase,K252a arrested cell cycle in G0/G1 phase,and NGF-β combined with K252a arrested cell cycle in S phase.Conclusion：NGF-β can enhance the proliferation of pancreatic carcinoma MIA PaCa-2 cells.

关 键 词：胰腺肿瘤 神经生长因子 细胞增殖 细胞周期 

分 类 号：R735.9[医药卫生—肿瘤] R730.2[医药卫生—临床医学]