裸鼠转基因结肠癌肝转移模型的建立  

Nude Mice Transgenic Hepatic Metastasis Model of Colon Cancer

作　　者：黎成金[1] 张宝明[1] 王烈[1] 涂小煌[1] 王羊 

机构地区：[1]南京军区福州总医院普通外科研究所,350025 [2]陕西省咸阳市中心医院普外科

出　　处：《肿瘤防治研究》2010年第2期150-153,共4页Cancer Research on Prevention and Treatment

基　　金：福建省自然科学基金资助项目(C0410045)

摘　　要：目的探讨裸鼠转基因结肠癌肝转移模型的建立方法。方法应用体外转染法和体内转染法建立裸鼠转基因结肠癌肝转移的动物模型。观察肝脏转移率和自然生存期;瘤组织作病理切片;实时荧光定量(Real-Time RT-PCR)检测CD基因表达,Western blot检测目的基因蛋白表达。结果体外转染法与体内转染法裸鼠肝脏转移率、自然生存期比较差异无统计学意义[100%(15/15),100%(15/15);(40.60±2.2297)d,(41.73±2.4919)d;P>0.05]。实时荧光定量PCR进行相对定量比较,体内转染法CD基因的表达活性低于体外转染法(P<0.05,t=2.758)。体内转染法瘤组织CD基因蛋白表达低于体外转染法。结论体外转染法建立裸鼠转基因结肠癌肝转移模型,目的基因在瘤组织中能更稳定、持续表达。Objective To explore the method to establish transgenic animal model for liver metastasis of colon cancer. Methods After establishing transgenic models for liver metastasis of human colon cancer Lovo cell which transfeced with CD gene in nude mice in vitro and in vivo, the rate of liver metastasis and the life span were detected between two groups. Pathological features were observed by HE stain. The RNA and protein expression of CD gene was analyzed by Real-time quantitative RT-PCR and Western-blot. Results No significant difference were observed between both groups about the rate of liver metastasis and the life span [100%（15/15）,100%（15/15）; （40.60±2.2297）d, （41.73±2.4919）d; P〉0.05）]. The RNA expression of CD gene in vivo was lower than that in vitro （P〈0.05,t=2.758）. The protein expression level of CD gene in the tumor tissue detected by westernblot was lower in vivo than that in vitro. Conclusion CD gene can more stably and persistently express in the Lovo cell transfected in vitro than that transfected in vivo.

关 键 词：结肠肿瘤 肝转移 基因 模型 裸鼠 

分 类 号：R735.35[医药卫生—肿瘤]