同一个体食管贲门双源癌中人乳头瘤病毒感染和p16^(INK4A)蛋白表达  

Detection of HPV16 Infection and p16^(INK4A) Expression in Concurrent Cancers from Esophagus and Gastric Cardia in Same High-incidence Area Patient

作　　者：丁广成[1,2] 王立东[1] 任景丽[3] 郭军辉[1] 袁翎[4] 郭涛[1] 

机构地区：[1]河南省食管癌重点开放实验室,郑州大学第一附属医院,郑州大学基础医学院,郑州450052 [2]郑州大学第五附属医院肿瘤放射治疗科 [3]郑州大学第二附属医院病理科 [4]河南省肿瘤医院肿瘤放射治疗科

出　　处：《肿瘤防治研究》2010年第2期172-175,共4页Cancer Research on Prevention and Treatment

基　　金：河南省医学科技重大攻关项目(WKJ2007-2-028);国家科技部863重大资助项目(2006AA02A403)

摘　　要：目的探讨河南食管癌高发区HPV感染与食管/贲门癌变的关系和p16INK4A蛋白表达的意义。方法利用聚合酶链式反应技术(PCR)检测17例双源癌患者食管鳞癌组织和贲门腺癌组织人乳头瘤病毒16型(HPV16)感染情况,应用免疫组织化学方法检测p16INK4A蛋白在17例双源癌鳞癌组织和腺癌组织中的表达。结果17例双源癌组织中8例食管鳞癌组织,5例贲门腺癌组织中检测到HPV16-DNA(47%vs.29%),且有2例患者食管鳞癌和贲门腺癌组织中同时检测到HPV16-DNA的存在。p16INK4A蛋白在两种组织中均有较高表达。在HPV相关的双源癌中,p16INK4A蛋白表达在腺癌组织中较鳞癌组织高(75%vs.25%,P<0.05)。结论河南食管癌高发区同一个体食管鳞癌和贲门腺癌中均有不同程度HPV感染,提示HPV可能是二者共同相关致病危险因素,高危型HPV16感染可能在河南食管癌高发区食管和贲门癌变过程中起重要作用。p16INK4A蛋白表达在腺癌组织中较鳞癌组织高,有可能成为HPV相关性贲门癌的一个筛查指标。Objective To investigate the correlations of human papillomavirus （HPV） infection and esophageal squamous cell cancer （ESCC）/ gastric cardia adenocarcinoma （GCA） and clinical significance of p16INK4A expression in the tumorigenesis of esophageal/gastric cardia epithelium by concurrent cancers from esophagus and gastric cardia in the same patient （CC） from Linzhou,a high-incidence area for SCC in Henan. Methods HPV16-E6 DNA and expressions of p16INK4A were detected by polymerase chain reaction （PCR） and ABC immunohistochemistry in 17 surgically resected specimens with SCC, 17 specimens with GCA. Results The positive rate of HPV16-E6 DNA was 47% （8/17） in SCC, and 29% （5/17） in GCA, respectively, and HPV16-E6 DNA was observed simultaneously in 2 specimens of SCC and 2 GCA,respectively. The immunohistochemical stain for p16INK4A protein was observed both in SCC and GCA tissues with different degrees. The expression of p16INK4A protein was significantly higher in GCA than that in SCC （75% vs.25%, P〈0.05）. Conclusion The consistent HPV infection in CC from the patients at same area suggests that HPV may be one of the common oncogenic factor for both SCC and GCA. HPV16, the high-risk type, may play an important role in the development of SCC and GCA. p16INK4A may be another candidate biomarker for screening of HPV-related GCA.

关 键 词：食管鳞癌 贲门腺癌 人乳头状瘤病毒 聚合酶链式反应 免疫组化 

分 类 号：R735.1[医药卫生—肿瘤] R735.2[医药卫生—临床医学]