RAPD和SRAP标记技术在苔藓植物亲缘关系研究中的比较分析  

The Comparative Analysis on the Study of Genetic Relationship of Bryophytes Based on RAPD and SRAP Markers

作　　者：张安世[1] 张为民[1] 邢智峰[1] 刘永英[1] 韦慧彦[1] 辛泽华[1] 

机构地区：[1]焦作师范高等专科学校生物系,河南焦作454003

出　　处：《中国农学通报》2010年第3期32-36,共5页Chinese Agricultural Science Bulletin

基　　金：河南省科技攻关项目"太行山苔藓植物资源调查及新型生物工程载体开发"(0624240019);河南省教育厅自然科学研究计划项目"河南省苔藓植物的系统发育研究"(2008C180002)

摘　　要：利用RAPD和SRAP标记技术研究11种苔藓植物的亲缘关系,并进行比较分析。结果表明:RAPD扩增的多态性比率达100%,SRAP扩增的多态性比率达96.9%,2种标记结果均显示了很高的多态性比率。利用SPSS11.5软件对RAPD和SRAP扩增结果进行了聚类分析,两者均与形态学分类基本一致,但与形态学结果也有一定的差异,这种差异主要表现在科以上植物种类之间。同时,2种分子标记的聚类结果之间也存在一定的差异,主要是对大叶凤尾藓和小牛舌藓全缘亚种的划分。因此,RAPD和SRAP都有一定局限性,需要多种标记方法相互结合、相互印证,才能得出客观的结果。RAPD and SRAP markers were applied to assess genetic relationship in 11 individuals of bryophytes.The results showed that both of techniques detected high proportion of polymorphic bands,which were 100%from RAPD and 96.9%from SRAP.By means of cluster analysis using SPSS 11.5 software,the resultant dendrograms from RAPD and SRAP analyses were showed the generally correspondence with morphological classification except for some species among family.Comparing the two techniques,there were also some differences between SRAP and RAPD markers,mainly in the classification of Fissidens grandifrons Brid.and Anomodon minor（Hedw.） Fuernr.ssp.integerrimus（Mitt.）Iwats.Therefore,RAPD and SRAP had certain limitations,and two molecular techniques should be combined in practice in order to obtain objective results.

关 键 词：苔藓 RAPD 亲缘关系 遗传多样性 

分 类 号：Q781[生物学—分子生物学]