日本血吸虫核糖体蛋白SjRPS4、SjRPL7 DNA疫苗对小鼠免疫保护作用研究  

Study on the immunoprotections of SjRPS4 and SjRPL7 DNA vaccines against Schistosoma japonicum infection in mice

作　　者：汪世平[1] 高冬梅[1] 何卓[1] 余路新[1] 陈秀春[1] 

机构地区：[1]中南大学湘雅医学院血吸虫病免疫与传播控制重点实验室,长沙410078

出　　处：《中国人兽共患病学报》2010年第2期97-100,共4页Chinese Journal of Zoonoses

基　　金：国家科技支撑计划(2009BAI78B05);国家重点基础研究发展(973)计划(No.2007CB513108);湖南省血吸虫病免疫与传播控制重点实验室;湖南省重点学科建设专项经费(No.07-985-2);中南大学研究生学位论文创新基金(2009bsxt016)联合资助

摘　　要：目的研究日本血吸虫核糖体蛋白SjRPS4、SjRPL7DNA疫苗对小鼠免疫保护作用。方法大量制备pcD-NA3.0/SjRPS4和pcDNA3.0/SjRPL7质粒DNA疫苗,昆明小鼠40只,随机均分为A、B、C和D组,A组为生理盐水(NS)组,每次肌肉注射100μL生理盐水;B组每只小鼠的股四头肌注射100μgpcDNA3.0裸质粒DNA;C组为pcDNA3.0/SjRPS4真核重组质粒组,每次肌肉注射质粒100μg/100μL;D组为pcDNA3.0/SjRPL7真核重组质粒组,每次肌肉注射质粒100μg/100μL。每隔2w同量加强免疫1次,共3次。末次免疫后第2w测定免疫各组血清特异性抗体效价后,经小鼠腹部皮肤人工感染20±1条日本血吸虫尾蚴。感染后第42d处死小鼠,计算减虫率和减卵率。结果与NS对照组比较,pcDNA3.0/SjRPS4和pcDNA3.0/SjRPL7免疫组小鼠均获得了较显著的减虫率、肝减卵率、肠减卵率、每雌子宫减卵率。统计学处理,均有显著性差异。结论pcDNA3.0/SjRPS4和pcDNA3.0/SjRPL7DNA疫苗对小鼠均有较强的免疫保护效果。The aim of this research was to study the immunoprotections of Schistosoma japonicurn （S. japonicum） DNA vaccines SjRPS4 and SjRPL7 in mice. Fourty Kunming mice were randomly divided into 4 groups （A, B, C and D）, and the pcDNA3. 0/SjRPS4 and pcDNA3. 0/SjRPL7 plasmid DNA vaccines were prepared for experiment. Mice in group A were intramuscularly injected with 100μL normal saline, whereas mice in group B were injected with 100 （g naked plasmid pcDNA3.0 into the quadriceps. Mice in groups C and D were injected with 100μg/100μL eukaryotic recombinant plasmids pcD- NA3.0/SjRPS4 and pcDNA3.0/SjRPL7 into the hind leg muscles respectively. The initial injections were followed by two sets of boosters at 2 weeks intervals. In addition, levels of the specific antibodies were detected 2 weeks after the last immunization and all mice were percutaneously infected with 20（1） S. japonicum cercariae on abdomen. Fourty-two days after the infection, all mice were killed to detect the worm reduction rate and the egg reduction rate. Significant differences of worm burden reduction rates, LEPG reduction rates, IEPG reduction rates and intrauterine eggs reduction rates were observed in both test group （group C and D）, comparing with the control groups （group A and B）. Results indicated that the DNA vaccines of pcDNA3.0/SjRPS4 and pcDNA3.0/SjRPL7 could induce strong protective immunity against S. japonicum in mice.

关 键 词：日本血吸虫 SjRPS4 SjRPL7 DNA疫苗 免疫保护 

分 类 号：R383.2[医药卫生—医学寄生虫学]