降解毒死蜱的高效复合微生物菌剂的制备及研究  

Preparating and Researching the Efficient Comple Microbial Community for Degradating Chlorpyrifos

作　　者：牛明芬[1] 刘知远[1] 李卓坪[1] 崔伟[1] 庞小平[1] 张迪[1] 

机构地区：[1]沈阳建筑大学市政与环境工程学院,沈阳110168

出　　处：《农业环境科学学报》2010年第2期381-385,共5页Journal of Agro-Environment Science

基　　金：陆地生态过程与生态安全重点实验室开放基金(1-1-2-5-0531);辽宁省自然基金(1-1-2-4-0586)

摘　　要：将菌株D1、D2分别与小克银汉按一定比例富集培养制成两种复合微生物菌剂(Ⅰ、Ⅱ),在充分供氧的条件下,通过气相色谱法研究两种复合菌剂的生长条件并筛选一种对毒死蜱有较好降解效果的高效复合微生物菌剂。结果表明,当接种量菌浓度为OD560=0.906时,两种菌剂生长的最适温度均为30℃,最适pH均为7.0;当毒死蜱浓度为100mg·L-1时,培养5d后两种复合菌剂的降解效率分别为81.21%和86.57%,当温度为30℃、pH为6.0～8.0、毒死蜱浓度为20mg·L-1时,对毒死蜱的降解效率最高。本研究工作为复合菌剂的大规模生产提供了理论参数,为利用微生物进行有机磷农药土壤修复提供了理论依据。The purpose of this research was to study the growth conditions of two complex microbial community Ⅰ, Ⅱ, which composed upon D1 and D2 between Cunninghamella respectively, and to select the one with better effect on degrading chlorpyrifos and to measure its degrading effect, under adequate oxygen condition. The result showed：when the concentration of microorganism is 0.906（OD560 value）, the optimum temperature of them were the same 30 ℃,and the optimum pH value were the same 7.0. When the chlorpyrifos concentration was 100 mg·L^-1, the degradation rate of them after 5 days were 81.21% and 86.57%. Under the condition of 30 ℃, pH varying between 6.0 and 8.0 and chlorpyrifos concentration of 20 mg· L^-1,the degradation rate was maximum. The results showed that its figures could offer theoretical figures in complex microbial community large-scale. Fermentation production and the bacterial might potentially be used in bioremediation of the soil which polluted by organic phosphorus pesticides.

关 键 词：毒死蜱 生物修复 高效复合微生物菌剂 拮抗作用 

分 类 号：X172[环境科学与工程—环境科学]