卡铂碳铁纳米球对大鼠移植性肝癌的靶向性研究  被引量:3

Targeted biodistribution of carboplatin-C Fe nanocage-loaded chitosan nanoparticles in rats with transplanted liver cancer

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作  者:郭跃华 严文辉 桂玲[2] 庞学斌 李富荣 鲍世韵 周汉新 

机构地区:[1]暨南大学第二临床医学院深圳市人民医院肝胆胰外科,广东深圳518020 [2]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030

出  处:《中华肿瘤防治杂志》2009年第23期1828-1831,共4页Chinese Journal of Cancer Prevention and Treatment

基  金:深圳市科技计划重点项目(200601016);广东省自然科学基金(04006966);广东省名医工程研究项目;深圳市科技计划项目(2002-K3-121)

摘  要:目的:观察卡铂碳铁纳米球(C-C&Fe-N)经肝动脉注射,结合体外磁场引导下,在移植性肝癌大鼠体内的靶向分布情况。方法:移植性肝癌大鼠40只为A组,健康大鼠40只为B组。肝动脉插管,注入C-C&Fe-N,以肿瘤(A组)或肝左叶(B组)为靶区,施加磁场30min(5000Gs)。分别于相应时间点,采集肿瘤或靶区肝、非靶区肝、肾、脾、肺和心组织标本,测定卡铂浓度,显微镜观察C-C&Fe-N在各脏器沉积情况。结果:A组靶区肿瘤组织卡铂峰浓度(Cmax)65.21μg/g,为B组靶区肝组织38.47μg/g的1.7倍,为A组非靶区肝组织14.36μg/g的4.5倍。48h时A组靶区肿瘤组织卡铂浓度7.27μg/g,为B组靶区肝组织3.11μg/g的2.3倍,为A组非靶区肝组织0.32μg/g的22.7倍。各时间点,A组肿瘤组织药物浓度均成倍高于B组靶区肝组织和两组非靶区肝组织,差异有统计学意义,P<0.05。A组靶区肿瘤组织卡铂AUC是906(μg.h)/g,为B组靶区肝组织421.34(μg.h)/g的2.2倍,为A组非靶区肝组织86.47(μg.h)/g的10.5倍。结论:C-C&Fe-N在体内显示出良好的磁靶向性和药物缓释性,并且对肿瘤组织具有更强的靶向性,能成倍提高肿瘤组织中的药物浓度,延长维持时间。OBJECTIVE:To observe the targeted biodistribution of Carboplatin-C &Fe Nanocage-loaded Chitosan Nanopartieles (C-C & Fe-N) injected by hepatic artery under magnetic field in rats with transplanted liver cancer. METHODS: Forty rats with transplanted liver cancer were composed of Group A. Forty normal rats were composed of Group B. Abdominal exposure was carried out through a midline incision, and a cannula was inserted into the hepatic artery and fixed. C-C & Fe-N was injected into hepatic artery. The tumors (Group A) and the left liver lobes (Group B) were under 5 000 Gs magnetic field as targeted region for 30 rain. At different injection time rats in each group were sacrificed respectively. The drug levels in tumor, targeted liver, non-targeted liver, kidney, spleen, lung and heart were measured by flameless atomic absorption spectrophotometry. The tissues were stained by HE to observe the aggregation of C-C & Fe-N. RESULTS: The Cmax of eaboplatin of targeted tumor in Group A (65.21 μg/g) was 1. 7 times higher than that of targeted liver in Group B (38.47 /μg/g), and 4.5 times higher than that of non-targeted liver in Group A(14.36 μg/g). The concentration of carboplatin of targeted tumor at 48 h in Group A (7.27 μg/g) was 2.3 times higher than that of targeted liver in Group B(3.11 μg/g), and 22. 7 times higher than that of non-targeted liver in Group A(0.32 μg/g). The drug concentrations of tumor at every time were significantly higher than those of targeted liver in Group B and those of nontargeted-liver in both groups (P〈0. 05). The area under curve of caboplatin of targeted tumor in Group A (906( μg · h)/g] was 2.2 times higher than that of targeted liver in Group B (421.34 (μg · h)/g], and 10.5 times higher than that of non-targeted liver in Group A (86.47 (μg·h)/g]. CONCLUSIONS: C C & Fe N shows remarkable magnetic target and delayed release in vivo, which are specially stronger to tumor than targeted liv

关 键 词:磁靶向 纳米球 卡铂 肝肿瘤 大鼠 

分 类 号:R735.7[医药卫生—肿瘤]

 

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