αvβ6整合素siRNA抑制人卵巢癌细胞体外及体内侵袭作用的研究  

Inhibition effects of integrin αvβ6 siRNA on invasiveness of human ovarian cancer cells in vitro and in vivo

作　　者：杜雪莲[1] 盛修贵[1] 宋趣清[1] 魏萍[1] 张小玲[1] 路春华[1] 

机构地区：[1]山东省肿瘤医院妇科,山东济南250117

出　　处：《中华肿瘤防治杂志》2009年第23期1832-1835,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金(Y2006C93)

摘　　要：目的:研究整合素αvβ6小干扰RNA对人卵巢癌HO-8910PM细胞侵袭生长的抑制作用。方法:以脂质体法将整合素αvβ6-siRNA(siRNA组)及无义寡核苷酸质粒(Neo组)转染人卵巢癌HO-8910PM细胞,通过实时定量PCR及蛋白质印迹试验,分别测定siRNA组、Neo组和空白对照组αvβ6整合素mRNA和蛋白的表达,MTT法和Transwell小室法分别测定其体外增殖能力和侵袭力。将各组细胞接种于裸小鼠皮下,观察移植瘤生长速度,并用免疫组化法检测整合素αvβ6的表达。结果:与Neo组和空白对照组相比较,siRNA组中αvβ6整合素mRNA和蛋白表达强度明显降低;转染的卵巢癌HO-8910PM细胞侵袭力明显下降(P<0.01);各组细胞体外增殖能力差异无统计学意义(P>0.05),但siRNA组细胞裸鼠皮下移植瘤生长速度明显低于其他两组,免疫组化显示,转染整合素αvβ6-siRNA的移植瘤中整合素αvβ6为阴性表达。结论:αvβ6整合素siRNA转染HO-8910PM细胞后,通过降低αvβ6整合素mRNA和蛋白表达水平,抑制其对细胞外基质的侵袭,进而抑制裸鼠移植瘤的生长。OBJECTIVE： To investigate the inhibition effect of ave6 siRNA on invasiveness of human ovarian cancer cells HO 8910PM. METHODS： With liposome as a vecter, integrin alphav beta6 siRNA and non oligonucleotide were transfected into HO 8910PM cells, which were then randomized to siRNA group, Neo group and control group. The expressions of integrin ave6 mRNA and protein were determined by quantitive RT-PCR and Western Blot, and the proliferation and invasion were measured by MTT and Transwell ventricle. Then cells were inoculated subcutaneously into the left flank of nude mice. The growth rate was measured and expressions of integrin ave6 in transplanted tumors of nude mice were detected by immunohistochemical method. RESULTS： Com pared with the Neo group and blank control, the expressions of integrin ave6 mRNA and protein in the siRNA group were obviously lower; the invasiveness of human ovarian cancer cells transfected by integrin αvβ6 siRNA was obviously decreased （P〈0. 01）. The proliferative activity of HO-8910PM cells in vitro was not affected by transfection with the alphav beta6 siRNA （P〉0.05）. Inhibition of αvβ6 expression in HO-8910PM reduced the cell invasiveness. Fur thermore, cells transfected with αvβ6 siRNA formed smaller tumor in transplanted tumors of nude mice than Neo and control group. CONCLUSION： Integrin alphav beta6 siRNA can inhibit invasiveness of human ovarian cancer cells, and may be of great benefit for finding rational approach in ovarian cancer therapy.

关 键 词：卵巢肿瘤/病理学 整合素ΑVΒ6 侵袭 SIRNA 转染 免疫组织化学 

分 类 号：R737.31[医药卫生—肿瘤]