CK19 mRNA在早期宫颈癌淋巴结微转移中的研究  

RESEARCH OF CK19 MRNA IN LYMPH NODE DETECTION OF EARLY STAGE CERVICAL CANCER WITH MICRO-METASTASES

作　　者：杨宏英[1] 卢玉波[1] 崔友红[1] 张红平[1] 

机构地区：[1]昆明医学院第三附属医院(云南省肿瘤医院),昆明650118

出　　处：《现代预防医学》2010年第5期951-953,共3页Modern Preventive Medicine

基　　金：2006年云南省教育厅资助项目(5Y0413C)

摘　　要：[目的]通过检测早期宫颈癌前哨淋巴结(SLN)中CK19 mRNA(cytokeratin 19)的表达,探讨CK19mRNA对宫颈癌淋巴结微转移检测的临床意义。[方法]①选择2007年12月～2008月12月昆明医学院第三附属医院(云南省肿瘤医院)妇科肿瘤临床研究中心早期宫颈癌初治患者30例。所有患者均行子宫广泛切除术和双盆腔淋巴结清扫术。②采用放射性核素联合亚甲蓝的方法识别SLN,逆转录-多聚酶链式反应(RT-PCR)方法检测SLN中CK19 mRNA的表达,与常规病理组织学方法比较,分析CK19 mRNA对早期宫颈癌淋巴结微转移检测的临床意义。[结果]①30例宫颈癌患者共检出SLN标本55枚,其中常规病理组织学方法检测SLN阳性率为14.5%(8/55)。经RT-PCR法检测SLNCK19mRNA阳性率为32.7%(18/55)。经常规病理组织学方法证实无癌转移的47枚淋巴结中,10枚CK19 mRNA表达阳性,阳性率为21.3%(10/47)。二者之间比较差异有统计学意义。②CK19 mRNA表达与病理类型有相关性(P﹤0.05)。[结论]与传统的病理组织学比较,采用RT-PCR技术检测淋巴结中CK19 mRNA表达能显著提高微转移的检出率。[Objective] Through detecting the expression of CK19 mRNA in sentinel lymph nodes of early stage cervical cancer,to investigate the role of CK19 mRNA in detection of micro-metastases.[Methods] ① 30 patients with cervical cancer were evaluated.All patients were treated by radical hysterectomy and lymphadenectomy at the Department of Gynecological Oncology in The Third Affiliated Hospital of Kunming Medical College（Yunnan Province Tumor Hospital） from Dec,2007 to Dec,2008.② Sentinel lymph nodes were identified with radioactivity isotope and methylene blue.Expression of CK19 mRNA was detected by RT-PCR in sentinel lymph node of the early stage cervical cancer.[Results] ① 55 sentinel lymph nodes were examined in 30 cases of cervical cancer.Histopathologic examinations demonstrated the presence of metastases in 8（14.5%） sentinel lymph nodes of 8 patients.However,RT-PCR detected the expression of CK19 in 18（32.7%） sentinel lymph nodes of 13 patients.RT-PCR identified the expression of CK19 in 10（21.3%） histologically uninvolved sentinel lymph nodes that were negative by HE staining,these samples were confirmed to have micro-metastases.Histopathology and RT-PCR were significantly different in detection of positive rate of micro-metastases（P﹤0.05）.② CK19 mRNA was significantly related to histopathology type（P﹤0.05）.[Conclusion] Detection of CK19 mRNA can help us to find the micro-metastases in lymph nodes early.

关 键 词：宫颈癌 SLN微转移 CK19MRNA RT-PCR 

分 类 号：R737.33[医药卫生—肿瘤]