组蛋白去乙酰化酶1 siRNA对HeLa细胞生长和凋亡的影响  

The Effect of Histone Deacetylases-1 siRNA on the Growth and Apoptosis of HeLa Cells

作　　者：李娜[1] 于世英[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤中心,武汉430030

出　　处：《华中科技大学学报（医学版）》2010年第1期47-49,58,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省科技攻关计划资助项目(No.2005AA304B07)

摘　　要：目的研究组蛋白去乙酰化酶1(histone deacetylases-1,HDAC-1)siRNA对宫颈癌HeLa细胞HDAC-1蛋白表达、细胞生长和凋亡的影响。方法HDAC-1 siRNA沉默HDAC-1蛋白;Western blot技术检测HDAC-1蛋白;MTT法和克隆形成实验检测细胞生长抑制情况;流式细胞仪检测细胞凋亡。结果HDAC-1 siRNA转染48 h可明显下调HeLa细胞HDAC-1蛋白表达;下调HDAC-1表达明显降低HeLa细胞克隆形成率,抑制细胞增殖;下调HDAC-1诱导HeLa细胞凋亡。结论HDAC-1 siRNA可能通过诱导凋亡抑制HeLa细胞生长。Objective To investigate the effect of histone deacetylases-l（HDAC-1）siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells. Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA. HDAC1 protein was detected by Western blot. The cell growth inhibition was assesses by MTT and clone forming assay. Apoptosis was measured by flow cytometry. Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously. The down-regulation of HDAC-1 significantly reduced the colony formation rate,inhibited cell proliferation and induced apoptosis in HeLa cells. Conclusion HDAC 1 siRNA may play an anti-proliferation role in HeLa cells by inducing apoptosis.

关 键 词：组蛋白去乙酰化酶1 SIRNA 子宫颈癌 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]