RNA干扰AKT2影响人胰腺癌细胞株裸鼠移植瘤对吉西他滨的敏感性  

The Effect of RNA Interference Suppressing the Expression of AKT2 on Sensitivity of Human Pancreatic Cancer Cell Line Xenograft in Nude Mice to Gemcitabine

作　　者：彭涛[1] 周峰[1] 杨鹏[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科,武汉430022

出　　处：《华中科技大学学报（医学版）》2010年第1期94-97,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的探讨RNA干扰AKT2对人胰腺癌细胞株裸鼠移植瘤对吉西他滨敏感性的影响。方法构建荷胰腺癌裸鼠模型,采用腹腔给药和瘤内注射方式,以吉西他滨配合AKT2 siRNA表达载体对荷瘤鼠进行联合干预治疗,对比观测裸鼠肿瘤生长情况,RT-PCR检测肿瘤细胞AKT2 mRNA的表达,免疫组织化学法检测肿瘤AKT2蛋白表达,DNA末端原位标记法(TUNEL法)检测细胞凋亡指数。结果化疗+AKT2-siRNA组移植瘤组织中AKT2 mRNA及AKT2蛋白表达明显低于空白对照组、化疗组、化疗+空白质粒组。化疗+AKT2-siRNA组瘤重、肿瘤体积显著低于其他各组。化疗+AKT2-siRNA组抑瘤率、凋亡指数显著高于其他各组。结论RNA干扰AKT2明显提高人胰腺癌细胞株对吉西他滨化疗的敏感性。Objective To study the effect of RNA interference suppressing the expression of AKT2 on sensitivity of human pancreatic cancer cell line xenograft in nude mice to gemcitabine. Methods The human pancreatic cancer cell implanted tumor model in the nude mice was established. By abdominal cavity administration and intratumoral injection,the mice bearing tumor were treated with gemcitabine in combination with vector of pAKT2-siRNA. Tumor growth of tumor tissues was observed,the AKT2 mRNA and protein expression levels in tumor tissues detected by RT-PCR and immunohistochemistry respectively,and tumor apoptosis measured by Tdt-mediated dUTP nick end labeling （TUNEL）. Results In chemotherapy＋ AKT2-siRNA group the expression of AKT2 mRNA and protein was significantly lower than in control group,chemotherapy group and chemotherapy＋ blank plasmid group. The tumor weight and tumor volume in chemotherapy＋ AKT2 siRNA group were significantly reduced as compared with those in other three groups. The inhibition rate and apoptosis rate in chemotherapy＋ AKT2-siRNA group were significantly higher than those in other three groups. Conclusion Sensitivity of human pancreatic cancer cell line to gemcitabine could be significantly improved by RNA interference suppressing the expression nf AKT2.

关 键 词：AKT2 胰腺癌 吉西他滨 

分 类 号：R735.9[医药卫生—肿瘤]