SLP-2荧光表达载体的构建及其在乳腺癌细胞MCF-7中的表达与定位  

Construction of pEGFP-C3-SLP-2 Plasmid and its Expression and Localization in MCF-7 Ceils

作　　者：王义军[1] 吴文韬[1] 徐甜[1] 向双林[1] 张健[1] 韩梅[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《激光生物学报》2010年第1期10-13,24,共5页Acta Laser Biology Sinica

基　　金：国家973资助项目(2006CB943506);长沙市科技厅计划项目(K071012-11)

摘　　要：人的SLP-2基因是一个新的肿瘤相关基因,它在多个癌组织里高表达,如食管鳞状细胞癌组织和肺癌组织。其中在乳腺癌组织的高表达和病人的存活率负相关,这说明该基因很可能在乳腺癌的发生中发挥重要作用。为了研究SLP-2基因在肿瘤里的功能,将人类SLP-2基因全长编码区定向连入pEGFP-C3质粒,使SLP-2蛋白可以与绿色荧光蛋白在乳腺癌细胞MCF-7内融合表达。而且细胞荧光实验发现SLP-2蛋白主要定位在MCF-7细胞的胞质内,这为进一步研究SLP-2基因在乳腺癌中的功能奠定了实验基础。Human stomatin like protein 2 （ SLP-2 ） is a novel cancer-related gene. Its protein is overexpressed in many human cancers, such as esophageal squamous cell carcinoma and lung cancer. In Breast cancer, high-level SLP-2 expression were associated with significantly decreased survival. To study the function of SLP-2 in these cancers, the coding region of human SLP-2 gene was successfully subcloned into the eukaryotic green fluorescent protein（GFP） expressing recombinant plasmids, pEGFP-C3. The vector was then transfected into MCF-7 cells for efficient expression. Moreover, results from fluorescent observation shows that GFP-SLP-2 mainly localized in cytoplasma, thus providing a useful clue for further research on the function of SLP-2 in breast cancer.

关 键 词：SLP-2 重组质粒 融合蛋白表达 细胞定位 

分 类 号：Q78[生物学—分子生物学]