京尼平交联的脱细胞牛心包生物支架材料的实验研究  

Experimental study of genipin crosslinked acellular bovine pericardium for biological scaffold materials

作　　者：陈平[1] 李新华[1] 邢万红[1] 

机构地区：[1]山西医科大学第二临床医院心胸外科,山西太原030001

出　　处：《中国医药导报》2010年第6期28-30,共3页China Medical Herald

基　　金：山西省科技攻关项目(20080311061-2);太原市科技发展项目(0904052)

摘　　要：目的:初步探讨新型生物交联剂京尼平对脱细胞牛心包基质作为支架材料的影响及意义,为心肌组织工程心脏补片的构建提供较理想的生物支架材料。方法:实验分为三组,A组(25块)为新鲜未脱牛心包组织,B组(25块)为脱细胞牛心包组织,C组(25块)为京尼平交联的脱细胞牛心包组织。采用光学显微镜、扫描电镜观察脱细胞效果和胶原纤维、弹力纤维改变;以组织厚度、热皱缩温度和抗拉力测试观察基质的物理性能变化。结果:光学显微镜、扫描电镜观察显示去污剂-酶消化法去细胞百分率达98.63%,能够有效脱除细胞,完整保留牛心包组织的胶原纤维和弹力纤维;组织厚度测试结果为,A组(3.8±1.0)mm,B组(3.8±1.2)mm,C组(4.0±1.0)mm;组织热皱缩温度测定结果为,A组(78.50±0.50)℃,B组(67.90±0.60)℃,C组(85.90±0.40)℃;组织抗拉力测试结果显示,经交联剂京尼平处理后C组最大载荷、最大应力、弹性模量、最大应变均高于A组,差异有统计学意义(P<0.05)。结论:经过新型生物交联剂京尼平交联的脱细胞牛心包最大载荷增加显著,可以弥补脱细胞牛心包力学性能的减少,作为较理想的心肌组织工程支架材料。Objective：To investigate the impact of biological crosslinking agent genipin on the changes of acellular bovine pericardium as a scaffold matrix,for the construction of the heart patch to provide a more ideal scaffold material in myocardial tissue engineering. Methods：Three groups were divided in the experiment,fresh bovine pericardium as group A （25）,acellular bovine pericardium as group B （25）,genipin crosslinked acellular bovine pericardium as group C （25）. The change of tissue collagen fiber and the acellular effect were observed by optical microscope and scanning electron microscopy. Tissue mechanical properties and shrinkage temperature were studied to observe changes in physical properties of the matrix. Results：Optical microscope and scanning electron microscopy showed that the acellular effect reached 98.63%,and the collagen fiber was very good preserved. Tissue thickness test results showed that group A was （3.8 ±1.0） mm,group B was （3.8±1.2） mm,group C was （4.0±1.0） mm; tissue shrinkage temperature test results showed that group A was （78.50±0.50）℃,group B was （67.90±0.60）℃,group C was （85.90±0.40）℃. Anti-pull test results indicated that max-load,max-stress,elastic modulus and max-straining of group C were higher than those of group A,they showed significant difference （ P〈0.05）. Conclusion：The max-load of genipin-fixed acellular bovine pericardium increases significantly,so it can make up for the mechanical properties of acellular bovine pericardium reduction. Therefore,it should be a promising biomaterial for fabricating the scaffold of tissue-engineered heart patch.

关 键 词：心肌组织工程 生物交联剂 脱细胞牛心包 

分 类 号：R-33[医药卫生]