抗苯磺隆猪殃殃乙酰乳酸合成酶的突变研究  

Study on Mutations in ALS of Resistance to Tribenuron-Methyl in Galium aparine L.

作　　者：孙健[1] 王金信[1] 张宏军[2] 刘君良[1] 卞圣楠[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]农业部农药检定所,北京100125

出　　处：《中国农业科学》2010年第5期972-977,共6页Scientia Agricultura Sinica

基　　金：国家"十一五"科技支撑计划(2006BAD08A09)

摘　　要：【目的】近几年,中国大部分冬小麦田猪殃殃(Galium aparineL.)用苯磺隆已无法有效控制。为明确猪殃殃对苯磺隆产生抗药性的分子机制,本试验从分子水平上开展研究,以初步明确乙酰乳酸合成酶(ALS)氨基酸序列的突变位点。【方法】通过对敏感和抗药性猪殃殃生物型ALS基因片段进行扩增、克隆和测序,比对2种生物型的ALS序列。【结果】与敏感生物型猪殃殃的ALS相比,抗药性猪殃殃生物型ALS有3个位点发生突变,其中位于高度保守区Domain B的第574位色氨酸被甘氨酸所取代。【结论】该位点的突变可能是猪殃殃对苯磺隆产生抗药性的主要原因。[Objective] In recent years, Galium aparine L. in most winter wheat fields in China could not be controlled by tribenuron-methyl. The objective of this study is to understand the molecular basis of the resistance mechanism to tribenuron-methyl in G. aparine and to find the specific mutation sites in amino acid sequence of acetolactate synthase （ALS） in the resistant biotype of G. aparine. [Method] Fragments encoding the ALS were amplified and cloned from G. aparine, susceptible （S） and resistant （R） biotypes to tribenuron-methyl, respectively, and sequenced subsequently. [Result] The result showed that the nucleotide sequence of R-biotype of G. aparine differed from that of the S biotype with three amino acid substitutions, of which, the amino acid substitution of Trp574 （TGG） to Gly （GGG） located in the highly conserved region Domain B. [Conclusion] The substitution of Trps74 might be responsible for the resistance to tribenuron-methyl in the R-biotype of G. aparine.

关 键 词：抗药性 猪殃殃 乙酰乳酸合成酶 基因突变 

分 类 号：S451[农业科学—植物保护]