银杏叶总黄酮对肝癌HepG2细胞增殖与凋亡的影响  

The effects of Ginkgo biloba leaf flavonoids and Quercetin on proliferation or Apoptosis of human liver cancer cell line HepG2

作　　者：杨利丽[1] 潘智芳[1] 耿秀芳[1] 刘红英[1] 李志坚[1] 

机构地区：[1]潍坊医学院,山东潍坊261041

出　　处：《中国优生与遗传杂志》2010年第2期27-28,20,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨银杏叶总黄酮体外对人肝癌细胞HepG2增殖与凋亡的影响。方法将银杏叶总黄酮作用于体外培养的人肝癌细胞HepG2,MTT法检测药物对HepG2细胞增殖的影响,缺口末端核苷标记(TUNEL)法检测药物对HepG2细胞凋亡的影响。结果银杏叶总黄酮对体外培养的人肝癌细胞HepG2的增殖效率下降,使凋亡细胞数增加(P<0.01),呈剂量依赖效应。结论银杏叶总黄酮对体外培养的人HepG2细胞增殖有抑制作用,并能诱导细胞凋亡。Objective：To investigate the effects of Ginkgo biloba leaf flavonoids on proliferation and apoptosis of human liver cancer cell line HepG2 in vitro. Methods：Human liver cancer cells line HepG2 cultured in vitro were treated with different concentrations of the flavonoids,and then the proliferation and apoptosis of the cells was detected by MTT and Tunnel assay. Resuls：Ginkgo biloba leaf flavonoids inhibited the proliferation of HepG2 cells line in a dose-dependent manner by MTT assay （P〈0.01）. And the apoptotic rate of the cells was also increased （P〈0.01）. Conclusion：Ginkgo biloba leaf flavonoids can inhibit the cell proliferation and promote apoptosis of human hepatoma cell line HepG2.

关 键 词：银杏叶总黄酮 肝癌 凋亡 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]