STK15的表达对NIH3T3细胞的影响  

Stimulating Effect of STK15 Gene on the Proliferation of NIH3T3 Fibroblasts

作　　者：朱焱[1,2] 王禄增[1] 杨威[1] 于洋[1] 王维[1] 董婉维[1] 汪瑛[1] 秦英[1] 郑志红[1] 

机构地区：[1]中国医科大学实验动物部,沈阳110001 [2]沈阳市公安医院,沈阳110001

出　　处：《中国比较医学杂志》2010年第2期17-20,I0002,共5页Chinese Journal of Comparative Medicine

基　　金：辽宁省科技计划项目(编号:2006408002-3)

摘　　要：目的构建pcDNA3.1-STK15表达质粒,探讨STK15基因对小鼠成纤维细胞(NIH3T3)的影响。方法构建pcDNA3.1-STK15质粒,将其转染NIH3T3,应用RT-PCR、免疫细胞化学和Western印迹方法检测STK15的表达;MTT法检测细胞增殖能力;Transwell检测细胞侵袭能力。结果转染pcDNA3.1-STK15质粒的NIH3T3细胞在48 h有STK15的表达,而且该细胞的增殖速度和穿透Matrigel胶的细胞数均明显高于对照组(P<0.05)。结论STK15基因具有增加细胞增殖和细胞侵袭力的功能,进而形成肿瘤。Objective To investigate the possible effect of STK15 gene on the NIH3T3 fibroblasts.Methods／pcDNA3.1-STK15 expression plasmid was constructed and transient transfected into NIH3T3 cells.The effects of STK15 gene on the NIH3T3 fibroblasts was examined by RT-PCR,immunocytochemistry and Western blot,respectively.The cell proliferation rate was measured by MTT.The capability of cell invasion was observed by Transwell.Results STK15 expression was significantly upregulated in pcDNA3.1-STK15 plasmid-transfected NIH3T3 cells.The MTT showed a significantly increased pcDNA3.1-STK15 plasmid-transfected NIH3T3 cell number invading through Matrigel filter compared with that of pcDNA3.1 vector transfected cells（P0.05）.Conclusions／STK15 gene can increase the capability of cell migration and invasion of NIH3T3 fibroblasts in vitro.It may suggest that STK15 gene expression may participate in the process of carcinogenesis.

关 键 词：STK15 细胞转染NIH3T3 

分 类 号：R-33[医药卫生]