Smad3基因敲除小鼠肾Smad2和p-Smad2表达的变化  

Change of Smad2 and p-Smad2 expression in the kidney of Smad3 knock-out mice

作　　者：吴婧[1] 徐健[2] 董静霞[2] 

机构地区：[1]长江大学医学院组织学与胚胎学系,荆州434000 [2]北京大学基础医学院解剖学与组织学胚胎学系,北京100091

出　　处：《解剖学杂志》2010年第1期26-28,共3页Chinese Journal of Anatomy

摘　　要：目的：观察Smad3基因敲除小鼠肾Smad2和p-Smad2表达的变化，探讨Smad3基因敲除小鼠肾是否有Smad2和p-Smad2代偿性增加。方法：4只Smad3基因敲除小鼠，10只野生型小鼠，应用免疫组织化学显色技术，检测Smad2和矿Smad2蛋白的定位和表达情况，并用Modc病理图像分析系统对图像进行半定量分析。结果：野生型小鼠肾Smad2蛋白在肾远端小管和肾集合管细胞胞质中有广泛弱表达，p-Smad2蛋白在肾远端小管和肾集合管细胞胞质、胞核中也有弱表达，而Smad3基因敲除小鼠的Smad2和p-Smad2的表达比野生型小鼠有显著升高。结论：Smad3基因敲除能引起小鼠肾Smad2和p-Smad2表达的代偿性增加。Objective： To study the changes of Smad2/p-Smad2 expression in the kidney of Smad3 knock-out mice. Methods： The expressions and localization of Smad2 and p-Smad2 proteins in the kidney of wide-type and Smad3 knockkout mice were examined by immunohistochemistry. Results： Smad2 protein was widely and weakly expressed in cytoplasm in distal renal tubules and collecting ducts of the wide-type mice, and p Smad2 protein was mainly expressed in the nuclei of collecting duets and distal tubules. The expression of Smad2 and p-Smad2 protein was significantly increased in the Smad3 knockout mice （P 〈 0.01）. Conclusion： Smad3 knock-out can cause a compensatory increase in the expression of Smad2 and p-Smad2 protein in the kidney of mice.

关 键 词：SMAD2 p-Smad2 Smad3基因敲除 小鼠 

分 类 号：R363.1[医药卫生—病理学] R780.2[医药卫生—基础医学]